中文版 | English
Title

Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections

Author
Corresponding AuthorYuan, Leyong; Hu, Xuejiao
Publication Years
2023
DOI
Source Title
EISSN
2076-0817
Volume12Issue:1
Abstract
Reliable diagnostics are necessary to identify influenza infections, and coronavirus disease 2019 (COVID-19) highlights the need to develop highly specific and sensitive viral detection methods to distinguish severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory pathogens to prevent their further spread. In this prospective study, 1070 clinical respiratory samples were collected from patients with acute respiratory infections from January 2019 to February 2021 to evaluate the diagnostic performance of a multiplex probe amplification (MPA) assay, designed to screen 18 pathogens, mainly those causing acute respiratory infections. Ninety-six positive samples and twenty negative samples for the 18 respiratory pathogens defined by the MPA assay and reverse transcription polymerase chain reaction (RT-PCR) were further confirmed by reference next-generation sequencing (NGS). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the MPA assay were 95.00%, 93.75%, 98.96% and 75.00%, respectively. Additionally, the co-infection rate for these positive samples were 25% (24/95). The MPA assay demonstrated a highly concordant diagnostic performance with NGS in the diagnosis of 18 respiratory pathogens and might play an important role in clinical respiratory pathogen diagnosis.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
WOS Research Area
Microbiology
WOS Subject
Microbiology
WOS Accession No
WOS:000915636100001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/430743
DepartmentSouthern University of Science and Technology Hospital
Affiliation
1.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Div Lab Med, Guangzhou 510080, Peoples R China
2.Southern Univ Sci & Technol Hosp, Dept Clin Lab, Shenzhen 518055, Peoples R China
Corresponding Author AffilicationSouthern University of Science and Technology Hospital
Recommended Citation
GB/T 7714
Li, Wenmin,Wang, Xiaoxiao,Cui, Wenhao,et al. Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections[J]. PATHOGENS,2023,12(1).
APA
Li, Wenmin,Wang, Xiaoxiao,Cui, Wenhao,Yuan, Leyong,&Hu, Xuejiao.(2023).Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections.PATHOGENS,12(1).
MLA
Li, Wenmin,et al."Clinical Evaluation of a Multiplex PCR Assay for Simultaneous Detection of 18 Respiratory Pathogens in Patients with Acute Respiratory Infections".PATHOGENS 12.1(2023).
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