Comparative transcriptome analyses of immune responses to LPS in peripheral blood mononuclear cells from the giant panda, human, mouse, and monkey

Li, Shun1; Li, Caiwu2; Chen, Lixiang1; Yang, Hua1; Ren, Xiaonan1; Xu, Chunhua1; Wu, Bin1; Wang, Chao1; Ling, Yun3; Shen, Yinzhong4; Lu, Hongzhou5; Liu, Weiping2; Zhou, Xiaohui1

2023-01-06

10.3389/fgene.2022.1053655

FRONTIERS IN GENETICS   Impact Factor & Quartile

1664-8021

Gram-negative bacteria are major pathogens that can cause illnesses in giant pandas. Lipopolysaccharides (LPS), components of Gram-negative bacteria, can activate immune responses in mammals (i.e., humans and mice) through recognition by toll-like receptors (TLRs). However, the giant pandas' immune response to LPS stimulation and the differences between the giant panda and other mammals are not fully known. In this study, we administrated peripheral blood mononuclear cells (PBMCs) from giant pandas, humans, C57BL/6 mice, and rhesus monkeys by LPS treatment at 6 h followed by RNA sequencing (RNA-seq), respectively, with control of non-stimulation. KEGG analyses of differentially expressed genes (DEGs) pathways indicated that LPS could activate the classic signaling pathway of NF-kappa B in PBMCs from those four tested species. Thus, similar to the other three species, NF-kappa B is an LPS-responsive regulator of innate immune responses in giant pandas. Furthermore, the expression patterns of adapter genes, inflammatory cytokine genes, chemokines, interferon genes, cytokine genes related to cell growth and development, costimulatory molecules, Th1/Th2 cytokine genes, Th17 cytokine genes, Th9, and Th22 cytokine genes were compared among giant pandas and three other species. Our data indicated that in addition to the similar expression patterns of certain genes among giant pandas and other species, the unique expression pattern response to LPS in giant pandas was also discovered. Furthermore, Th9, Th17, and Th22 cells might be involved in the response to LPS in giant pandas at this tested time point. This study reveals that LPS-induced immune responses have different sensitivities and response timelines in giant pandas compared with other mammals. This study facilitates further understanding of the role of the TLR signaling pathway and the immune system in giant pandas, which might be helpful for disease prevention and protection.

LPS immune responses comparative transcriptome analyses PBMCs giant panda

SCI

English

Corresponding

Conservation Biology of Rare Animals in the Giant Panda National Park (an open project of the Key Laboratory of State Forestry and Grassland Administration) Zhonghu R D (2019)["KLSFGAGP 2020.008","62"] ; Project of Shanghai Municipal Health Commission[20204Y0335] ; Sub project of Science and Technology Program of Shanghai Municipal Commission of Science and Technology[21140900202]

Genetics & Heredity

Genetics & Heredity

WOS:000918751600001

FRONTIERS MEDIA SA

Web of Science

1.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Anim Model, Shanghai, Peoples R China
2.China Conservat & Res Ctr Giant Panda, Key Lab State Forestry & Grassland Adm Conservat B, Dujiangyan, Sichuan, Peoples R China
3.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Infect Dis, Shanghai, Peoples R China
4.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Infect Dis & Immunol, Shanghai, Peoples R China
5.Southern Univ Sci & Technol, Hosp Affiliated 2, Shenzhen, Guangdong, Peoples R China

Li, Shun,Li, Caiwu,Chen, Lixiang,et al. Comparative transcriptome analyses of immune responses to LPS in peripheral blood mononuclear cells from the giant panda, human, mouse, and monkey[J]. FRONTIERS IN GENETICS,2023,13.

Li, Shun.,Li, Caiwu.,Chen, Lixiang.,Yang, Hua.,Ren, Xiaonan.,...&Zhou, Xiaohui.(2023).Comparative transcriptome analyses of immune responses to LPS in peripheral blood mononuclear cells from the giant panda, human, mouse, and monkey.FRONTIERS IN GENETICS,13.

Li, Shun,et al."Comparative transcriptome analyses of immune responses to LPS in peripheral blood mononuclear cells from the giant panda, human, mouse, and monkey".FRONTIERS IN GENETICS 13(2023).