Photoswitchable hemithioindigo inspired copper ion selective sensing with excellent selectivity and versatile operational modes

Chen，Qinghan1,2; Wang，Yifu2; Zhai，Jingying3,4; Xie，Xiaojiang2,4

2023-04-15

10.1016/j.snb.2023.133437

SENSORS AND ACTUATORS B-CHEMICAL   Impact Factor & Quartile

0925-4005

Copper is an essential element in living organisms and the monitoring of copper ions in aquatic environments and biological systems is of great significance. Inspired by a family of visible light responsive photoswitchable compounds, we report here a fluorescent probe (HTI-Q) for Cu with excellent selectivity, high sensitivity, and a limit of detection (LOD) of ca. 0.02 μM. While the central carbon-carbon double bond of HTI-Q is photoisomerizable between the Z and E configurations, it is locked upon binding with Cu into the E configuration, resulting in a large bathochromic shift (102 nm) and ratiometric fluorescence changes. Different sensing modes were demonstrated including in mixed solvents, a two-phase sensing system containing cation exchanger, and fluorescent nanoprobes containing a reference dye. The absorbance change of HTI-Q upon 470 nm light illumination also depended on the concentration of Cu. HTI-Q-based sensors were successfully applied to determine Cu in real water samples with excellent recovery. Further, fluorescent nanoprobes incorporating HTI-Q were successfully applied to image endolysosomal Cu changes upon external stimulation.

Cell imaging Copper Fluorescent probe Hemithioindigo Lysosomes Photoswitch

English

Corresponding

CHEMISTRY

2-s2.0-85147122392

Scopus

1.School of Chemistry and Chemical Engineering,Harbin Institute of Technology,Harbin,150001,China
2.Department of Chemistry,Southern University of Science and Technology,Shenzhen,518055,China
3.Academy for Advanced Interdisciplinary Studies,Southern University of Science and Technology,Shenzhen,518055,China
4.Guangdong Provincial Key Laboratory of Advanced Biomaterials,Southern University of Science and Technology,Shenzhen,518055,China

Chen，Qinghan,Wang，Yifu,Zhai，Jingying,et al. Photoswitchable hemithioindigo inspired copper ion selective sensing with excellent selectivity and versatile operational modes[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,381.

Chen，Qinghan,Wang，Yifu,Zhai，Jingying,&Xie，Xiaojiang.(2023).Photoswitchable hemithioindigo inspired copper ion selective sensing with excellent selectivity and versatile operational modes.SENSORS AND ACTUATORS B-CHEMICAL,381.

Chen，Qinghan,et al."Photoswitchable hemithioindigo inspired copper ion selective sensing with excellent selectivity and versatile operational modes".SENSORS AND ACTUATORS B-CHEMICAL 381(2023).