中文版 | English
Title

Molecular basis of the plant ROS1-mediated active DNA demethylation

Author
Corresponding AuthorDu, Jiamu
Publication Years
2023-01-09
DOI
Source Title
ISSN
2055-026X
EISSN
2055-0278
Volume9Issue:2Pages:271-279
Abstract

Active DNA demethylation plays a crucial role in eukaryotic gene imprinting and antagonizing DNA methylation. The plant-specific REPRESSOR OF SILENCING 1/DEMETER (ROS1/DME) family of enzymes directly excise 5-methyl-cytosine (5mC), representing an efficient DNA demethylation pathway distinct from that of animals. Here, we report the cryo-electron microscopy structures of anArabidopsisROS1 catalytic fragment in complex with substrate DNA, mismatch DNA and reaction intermediate, respectively. The substrate 5mC is flipped-out from the DNA duplex and subsequently recognized by the ROS1 base-binding pocket through hydrophobic and hydrogen-bonding interactions towards the 5-methyl group and Watson- Crick edge respectively, while the different protonation states of the bases determine the substrate preference for 5mC over T:G mismatch. Together with the structure of the reaction intermediate complex, our structural and biochemical studies revealed the molecular basis for substrate specificity, as well as the reaction mechanism underlying 5mC demethylation by the ROS1/ DME family of plant-specific DNA demethylases.

URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
Funding Project
Shenzhen Science and Technology Program[
WOS Research Area
Plant Sciences
WOS Subject
Plant Sciences
WOS Accession No
WOS:000911292100002
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/472484
DepartmentSUSTech-Peking University National Institute of Plants and Food
生命科学学院
生命科学学院_生物系
生命科学学院_前沿生物技术研究院
Affiliation
1.Southern Univ Sci & Technol, Key Lab Mol Design Plant Cell Factory Guangdong Hi, Inst Plant & Food Sci, Sch Life Sci, Shenzhen, Peoples R China
2.Shenzhen Univ Med Sch, Int Canc Ctr, Dept Biochem & Mol Biol, Shenzhen, Peoples R China
3.Univ Calif Berkeley, Calif Inst Quantitat Biosci QB3, Berkeley, CA USA
4.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA USA
5.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Beijing Adv Innovat Ctr Struct Biol, Minist Educ Key Lab Prot Sci, Beijing, Peoples R China
6.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen, Peoples R China
7.Chinese Acad Agr Sci, Ctr Adv Bioind Technol, Beijing, Peoples R China
First Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Corresponding Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
First Author's First AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Recommended Citation
GB/T 7714
Du, Xuan,Yang, Zhenlin,Xie, Guohui,et al. Molecular basis of the plant ROS1-mediated active DNA demethylation[J]. Nature Plants,2023,9(2):271-279.
APA
Du, Xuan.,Yang, Zhenlin.,Xie, Guohui.,Wang, Changshi.,Zhang, Laixing.,...&Du, Jiamu.(2023).Molecular basis of the plant ROS1-mediated active DNA demethylation.Nature Plants,9(2),271-279.
MLA
Du, Xuan,et al."Molecular basis of the plant ROS1-mediated active DNA demethylation".Nature Plants 9.2(2023):271-279.
Files in This Item:
File Name/Size DocType Version Access License
2023.Nat.Plants.pdf(6804KB) Restricted Access--
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Export to Excel
Export to Csv
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Du, Xuan]'s Articles
[Yang, Zhenlin]'s Articles
[Xie, Guohui]'s Articles
Baidu Scholar
Similar articles in Baidu Scholar
[Du, Xuan]'s Articles
[Yang, Zhenlin]'s Articles
[Xie, Guohui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Du, Xuan]'s Articles
[Yang, Zhenlin]'s Articles
[Xie, Guohui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.