中文版 | English
Title

A Dueling-Competent Signal-Sensing Module Guides Precise Delivery of Cargo Proteins into Target Cells by Engineered Pseudomonas aeruginosa

Author
Corresponding AuthorDong, Tao
Publication Years
2023-02-17
DOI
Source Title
ISSN
2161-5063
EISSN
2161-5063
Volume12Issue:2Pages:360-368
Abstract

To recognize and manipulate a specific microbe of a crowded community is a highly challenging task in synthetic biology. Here we introduce a highly selective protein delivery platform, termed DUEC, which responds to direct contact of attacking cells by engineering the tit-for-tat/dueling response of H1-T6SS (type VI secretion system) in Pseudomonas aeruginosa. Using a Cre-recombinase-dependent reporter, we screened H1-T6SS-secreted substrates and developed Tse6N as the most effective secretion tag for Cre delivery. DUEC cells can discriminately deliver the Tse6N-Cre cargo into the cytosol of T6SS+ but not T6SS- Vibrio cholerae cells. DUEC could also deliver a nuclease cargo, Tse6N-NucSe1, to selectively kill provoking cells in a mixed community. These data demonstrate that the DUEC cell not only is a prototypical physical-contact sensor and delivery platform but also may be coupled with recombination-based circuits with the potential for complex tasks in mixed microbial communities.

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Key R&D Program of China[
WOS Research Area
Biochemistry & Molecular Biology
WOS Subject
Biochemical Research Methods
WOS Accession No
WOS:000921766000001
Publisher
Scopus EID
2-s2.0-85146860111
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/475014
DepartmentSchool of Life Sciences
生命科学学院_基础免疫与微生物学系
Affiliation
1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China
2.Southern Univ Sci & Technol, Sch Life Sci, Dept Immunol & Microbiol, Shenzhen 518055, Peoples R China
Corresponding Author AffilicationSchool of Life Sciences
Recommended Citation
GB/T 7714
Wu, Li-Li,Yan, Shuangquan,Pei, Tong-Tong,et al. A Dueling-Competent Signal-Sensing Module Guides Precise Delivery of Cargo Proteins into Target Cells by Engineered Pseudomonas aeruginosa[J]. ACS Synthetic Biology,2023,12(2):360-368.
APA
Wu, Li-Li.,Yan, Shuangquan.,Pei, Tong-Tong.,Tang, Ming-Xuan.,Li, Hao.,...&Dong, Tao.(2023).A Dueling-Competent Signal-Sensing Module Guides Precise Delivery of Cargo Proteins into Target Cells by Engineered Pseudomonas aeruginosa.ACS Synthetic Biology,12(2),360-368.
MLA
Wu, Li-Li,et al."A Dueling-Competent Signal-Sensing Module Guides Precise Delivery of Cargo Proteins into Target Cells by Engineered Pseudomonas aeruginosa".ACS Synthetic Biology 12.2(2023):360-368.
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