Engineering orthotopic tumor spheroids with organ-specific vasculatures for local chemoembolization evaluation

Gao, Yanan1; Xiao, Jingyu1; Chen, Zijian1,2; Ma, Yutao1; Liu, Xiaoya1; Yang, Dishuang1; Leo, Hwa Liang2; Yu, Hanry3,4,5,6; Kong, Jian7; Guo, Qiongyu1

2023

10.1039/d2bm01632j

Biomaterials Science   Impact Factor & Quartile

2047-4830

2047-4849

Developing a three-dimensional (3D) in vitro tumor model with vasculature systems suitable for testing endovascular interventional therapies remains a challenge. Here we develop an orthotopic liver tumor spheroid model that captures the organ-level complexity of vasculature systems and the extracellular matrix to evaluate transcatheter arterial chemoembolization (TACE) treatment. The orthotopic tumor spheroids are derived by seeding HepG2 cell colonies with controlled size and location surrounding the portal triads in a decellularized rat liver matrix and are treated by clinically relevant drug-eluting beads embolized in a portal vein vasculature while maintaining dynamic physiological conditions with nutrient and oxygen supplies through the hepatic vein vasculature. The orthotopic tumor model exhibits strong drug retention inside the spheroids and embolization location-dependent cellular apoptosis responses in an analogous manner to in vivo conditions. Such a tumor spheroid model built in a decellularized scaffold containing organ-specific vasculatures, which closely resembles the unique tumor microenvironment, holds the promise to efficiently assess various diagnostic and therapeutic strategies for endovascular therapies.

SCI

English

First ; Corresponding

Key-Area Research and Development Program of Guangdong Province[2019B010941001] ; National Natural Science Foundation of China[81971764] ; Guangdong Research Fund[2019ZT08Y191] ; Innovation Committee of Shenzhen Municipality[JCYJ20200109141638004] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009] ; Special Fund for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District[CXY202001-02]

Materials Science

Materials Science, Biomaterials

WOS:000922143400001

ROYAL SOC CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen Key Lab Smart Healthcare Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China
2.Natl Univ Singapore, Dept Biomed Engn, Singapore 117583, Singapore
3.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore 117593, Singapore
4.Natl Univ Singapore, Mechanobiol Inst, Singapore 117411, Singapore
5.Agcy Sci Technol & Res, Inst Bioengn & Nanotechnol, Singapore 138669, Singapore
6.Singapore MIT Alliance Res & Technol, Singapore 138602, Singapore
7.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Dept Intervent Radiol,Clin Med C, Shenzhen 518020, Guangdong, Peoples R China

Gao, Yanan,Xiao, Jingyu,Chen, Zijian,et al. Engineering orthotopic tumor spheroids with organ-specific vasculatures for local chemoembolization evaluation[J]. Biomaterials Science,2023.

Gao, Yanan.,Xiao, Jingyu.,Chen, Zijian.,Ma, Yutao.,Liu, Xiaoya.,...&Guo, Qiongyu.(2023).Engineering orthotopic tumor spheroids with organ-specific vasculatures for local chemoembolization evaluation.Biomaterials Science.

Gao, Yanan,et al."Engineering orthotopic tumor spheroids with organ-specific vasculatures for local chemoembolization evaluation".Biomaterials Science (2023).