Low-Dose NIR-II Preclinical Bioimaging Using Liposome-Encapsulated Cyanine Dyes

Gao, Duyang1; Luo, Zichao2,3; He, Yang4; Yang, Lixiang4; Hu, Dehong1; Liang, Yongye5; Zheng, Hairong1; Liu, Xiaogang2,3; Sheng, Zonghai1

2023

10.1002/smll.202206544

Small   Impact Factor & Quartile

1613-6810

1613-6829

Fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) provides a powerful tool for in vivo structural and functional imaging in deep tissue. However, the lack of biocompatible contrast agents with bright NIR-II emission has hindered its application in fundamental research and clinical trials. Herein, a liposome encapsulation strategy for generating ultrabright liposome-cyanine dyes by restricting dyes in the hydrophobic pockets of lipids and inhibiting the aggregation, as corroborated by computational modeling, is reported. Compared with free indocyanine green (ICG, an US Food and Drug Administration-approved cyanine dye), liposome-encapsulated ICG (S-Lipo-ICG) shows a 38.7-fold increase in NIR-II brightness and enables cerebrovascular imaging at only one-tenth dose over a long period (30 min). By adjusting the excitation wavelength, two liposome-encapsulated cyanine dyes (S-Lipo-ICG and S-Lipo-FD1080) enable NIR-II dual-color imaging. Moreover, small tumor nodules (2-5 mm) can be successfully distinguished and removed with S-Lipo-ICG image-guided tumor surgery in rabbit models. This liposome encapsulation maintains the metabolic pathway of ICG, promising for clinical implementation.

cyanine dyes liposomes preclinical imaging second near-infrared (NIR-II) fluorescence imaging

SCI

English

Others

Natural Science Foundation of China["81901812","82171958","81771906","91859117","82027803","81927807","92159304"] ; CAS Key Laboratory of Health Informatics[2011DP173015] ; Guangdong Basic and Applied Basic Research Fund["2022A1515010384","2022A1515140102"] ; Key Laboratory for Magnetic Resonance and Multimodality Imaging of Guangdong Province[2020B1212060051] ; Science and Technology Key Project of Shenzhen["JCYJ20190812163614809","JCYJ20200109114612308","JCYJ20210324120011030"] ; Shenzhen Key Laboratory of Ultrasound Imaging and Therapy[ZDSYS201802061806314]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000921591400001

WILEY-V C H VERLAG GMBH

Web of Science

1.Chinese Acad Sci, Inst Biomed & Hlth Engn, Shenzhen Inst Adv Technol,Shenzhen Key Lab Ultraso, Paul C Lauterbur Res Ctr Biomed Imaging,CAS Key La, Shenzhen 518055, Peoples R China
2.Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
3.Natl Univ Singapore, Ctr NanoMed, Singapore 117543, Singapore
4.BayRay Innovat Ctr, Shenzhen Bay Lab, Shenzhen 518055, Peoples R China
5.Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China

Gao, Duyang,Luo, Zichao,He, Yang,et al. Low-Dose NIR-II Preclinical Bioimaging Using Liposome-Encapsulated Cyanine Dyes[J]. Small,2023.

Gao, Duyang.,Luo, Zichao.,He, Yang.,Yang, Lixiang.,Hu, Dehong.,...&Sheng, Zonghai.(2023).Low-Dose NIR-II Preclinical Bioimaging Using Liposome-Encapsulated Cyanine Dyes.Small.

Gao, Duyang,et al."Low-Dose NIR-II Preclinical Bioimaging Using Liposome-Encapsulated Cyanine Dyes".Small (2023).