O-GalNAc glycosylation affects the immunogenicity of the receptor-binding domain (RBD) of SARS-CoV-2 spike protein

Rong, Yongheng1; Wang, Xingyun2; Mao, Weian1; Chen, Min1; Wang, Shengjun3; Wang, Peng George2; He, Yunjiao2; Kong, Yun1

2023-02-09

10.1039/d2cc06583e

CHEMICAL COMMUNICATIONS   Impact Factor & Quartile

1359-7345

1364-548X

The spike protein of SARS-CoV-2 has been widely used as an effective vaccine immunogen, although some limitations still remain. Herein, O-GalNAc glycosylated RBD (Tn-RBD) was synthesized as an antigen via in vitro glycosylation reactions. The inhibition ability against hACE2 binding of antibodies induced with Tn-RBD was 30-40% increased compared to that induced with RBD. This result implies that Tn-glycosylation might play important roles in the immunogenicity of the RBD protein, which should be considered in the design of novel vaccines to fight against COVID-19.

SCI

English

NI Journal Papers ; NI论文

Corresponding

Shandong Province Key Research and Develop Program["2019GSF107048","2020CXG-C010601"] ; National Natural Science Foundation of China["31500648","31971213"] ; Guangdong Basic, Applied Basic Research Foundation[2019A1515010647] ; Open Projects Fund of Shandong Key Laboratory of Carbohydrate Chemistry and Glyco-biology, Shandong University[2019CCG08] ; Shenzhen Science and Technology Program[KQTD20200909113758004] ; COVID-19 Prevention and Control Special Project of Guangdong Education Department[2020KZDZX1181]

Chemistry

Chemistry, Multidisciplinary

WOS:000920163600001

ROYAL SOC CHEMISTRY

CHEMISTRY

Web of Science

1.Shandong Univ, Natl Glycoengn Res Ctr, Shandong Key Lab Carbohydrate Chem & Glycobiol, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China
2.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
3.Univ Hlth & Rehabil Sci, Sch Hlth & Life Sci, Qingdao 266071, Peoples R China

Rong, Yongheng,Wang, Xingyun,Mao, Weian,et al. O-GalNAc glycosylation affects the immunogenicity of the receptor-binding domain (RBD) of SARS-CoV-2 spike protein[J]. CHEMICAL COMMUNICATIONS,2023,59(13).

Rong, Yongheng.,Wang, Xingyun.,Mao, Weian.,Chen, Min.,Wang, Shengjun.,...&Kong, Yun.(2023).O-GalNAc glycosylation affects the immunogenicity of the receptor-binding domain (RBD) of SARS-CoV-2 spike protein.CHEMICAL COMMUNICATIONS,59(13).

Rong, Yongheng,et al."O-GalNAc glycosylation affects the immunogenicity of the receptor-binding domain (RBD) of SARS-CoV-2 spike protein".CHEMICAL COMMUNICATIONS 59.13(2023).