Comparative studies on mannan and imiquimod induced experimental plaque psoriasis inflammation in inbred mice

Wu, Huimei1,2; Ou, Jiaxin1,3,4; Li, Kangxin1,5; Wang, Tingting1,6; Nandakumar, Kutty Selva1,7

2023

10.1093/cei/uxad004

CLINICAL AND EXPERIMENTAL IMMUNOLOGY   Impact Factor & Quartile

0009-9104

1365-2249

Psoriasis is a genetically determined, environmentally triggered, immune system-mediated autoimmune disease. Different animal models are needed to investigate the complex pathological mechanisms underlying this disease. Therefore, we established mannan-induced psoriasis model and compared with the most commonly used imiquimod-induced psoriasis in terms of disease, induction of innate immune cells, expression of cytokines, and the effect of dexamethasone treatment. Mannan significantly induced more severe psoriasis with better disease relapsing feature than imiquimod (IMQ). As determined by immunohistochemistry, IMQ induced significantly more infiltration of CD11c(+) and F4/80(+) cells than mannan in the skin. However, cytometric analysis showed a significant increase in the percentage of Gr-1(+) neutrophils in the spleen and lymph nodes as well as F4/80(+) macrophages in the spleen after mannan exposure. Variation in the percentage of significantly increased V gamma 4 T cells was also found to be dependent on the lymphoid organs tested. However, there is a clear difference between these models in terms of expression of certain cytokine genes: IL-22, IL-23, IL-17E, and IL-17F were expressed more predominantly in mannan-induced inflammation, while IL-6 and IL-17A expressions were significantly higher in IMQ model. Interestingly, dexamethasone treatment strongly reduced epidermal thickness and histological scores induced by mannan than IMQ. Despite inducing psoriasis-like inflammation, certain differences and similarities were observed in the immune responses induced by mannan and IMQ. However, mannan-induced psoriasis model is relatively more simple, economical and less harmful to mice with an increased possibility to develop a chronic psoriasis model by exposing mice to mannan.

psoriasis innate immune cells gamma delta T cells IL-23 IL-17 axis mannan IMQ

SCI

English

Others

Southern Medical University, Guangzhou, China["C1034211","C1051004"]

Immunology

Immunology

WOS:000935394800001

OXFORD UNIV PRESS

IMMUNOLOGY

Web of Science

1.Southern Med Univ, Karolinska Inst, Sch Pharmaceut Sci, United Med Inflammat Ctr, Guangzhou, Peoples R China
2.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China
3.Southern Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Guangzhou, Peoples R China
4.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou Key Lab Drug Res Emerging Virus Prevent, Guangzhou, Peoples R China
5.Southern Med Univ, Affiliated Hosp 5, Dept Endocrinol, Guangzhou, Peoples R China
6.Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou, Guangdong, Peoples R China
7.Halmstad Univ, Sch Business Innovat & Sustainabil FIH, Dept Environm & Biosci, Halmstad, Sweden

Wu, Huimei,Ou, Jiaxin,Li, Kangxin,et al. Comparative studies on mannan and imiquimod induced experimental plaque psoriasis inflammation in inbred mice[J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY,2023.

Wu, Huimei,Ou, Jiaxin,Li, Kangxin,Wang, Tingting,&Nandakumar, Kutty Selva.(2023).Comparative studies on mannan and imiquimod induced experimental plaque psoriasis inflammation in inbred mice.CLINICAL AND EXPERIMENTAL IMMUNOLOGY.

Wu, Huimei,et al."Comparative studies on mannan and imiquimod induced experimental plaque psoriasis inflammation in inbred mice".CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2023).