| Title | The fluorescence quenching mechanism of tetrazine-functionalized fluorogenic labels with integrated pi-conjugations: internal conversion to a dark state |
| Author | |
| Corresponding Author | Liu, Xiaogang |
| Publication Years | 2023
|
| DOI | |
| Source Title | |
| EISSN | 2052-1537
|
| Abstract | Tetrazine-based fluorogenic labels have been extensively applied to the studies of living systems due to their excellent selectivity, rapid reaction kinetics, and unique fluorogenicity. However, the fluorescence quenching mechanism in many precursor dyes remains controversial, impeding rational molecular engineering to optimize their fluorescence enhancement ratios. Herein, employing quantum chemical calculations, we established a quenching mechanism - internal conversion to a dark state (ICDS), to rationalize the fluorogenicity in tetrazine-functionalized labels with integrated pi-conjugations. We show that the ICDS efficiency is negatively correlated with the energy gap between these labels' first excited state (the dark state) and the second excited state (the bright state). Based on this correlation, we further proposed molecular design strategies for developing biocompatible long-wavelength fluorophores with improved fluorogenicity. |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | Others
|
| Funding Project | Ministry of Education Singapore[MOE-MOET2EP1020-0007]
; Agency for Science, Technology and Research (A*STAR, Singapore)[A2083c0051]
; National Natural Science Foundation of China[12204229]
|
| WOS Research Area | Chemistry
; Materials Science
|
| WOS Subject | Chemistry, Multidisciplinary
; Materials Science, Multidisciplinary
|
| WOS Accession No | WOS:000928682600001
|
| Publisher | |
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:0
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/489977 |
| Department | Department of Electrical and Electronic Engineering |
| Affiliation | 1.Singapore Univ Technol & Design, Sci Math & Technol Cluster, 8 Somapah Rd, Singapore 487372, Singapore 2.Southern Univ Sci & Technol, Inst Nanosci & Applicat, Shenzhen 518055, Peoples R China 3.Southern Univ Sci & Technol, Dept Elect & Elect Engn, Shenzhen 518055, Peoples R China |
| Recommended Citation GB/T 7714 |
Shen, Tianruo,Zhang, Wenda,Yadav, Priya,et al. The fluorescence quenching mechanism of tetrazine-functionalized fluorogenic labels with integrated pi-conjugations: internal conversion to a dark state[J]. MATERIALS CHEMISTRY FRONTIERS,2023.
|
| APA |
Shen, Tianruo,Zhang, Wenda,Yadav, Priya,Sun, Xiao Wei,&Liu, Xiaogang.(2023).The fluorescence quenching mechanism of tetrazine-functionalized fluorogenic labels with integrated pi-conjugations: internal conversion to a dark state.MATERIALS CHEMISTRY FRONTIERS.
|
| MLA |
Shen, Tianruo,et al."The fluorescence quenching mechanism of tetrazine-functionalized fluorogenic labels with integrated pi-conjugations: internal conversion to a dark state".MATERIALS CHEMISTRY FRONTIERS (2023).
|
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment