Metal-Organic Frameworks in Microfluidics Enable Fast Encapsulation/Extraction of DNA for Automated and Integrated Data Storage

Mao, Cuiping1; Wang, Shuchen1; Li, Jiankai1; Feng, Zhuowei1; Zhang, Tong2; Wang, Rui2; Fan, Chunhai3; Jiang, Xingyu1

2023-02-01

10.1021/acsnano.2c11241

ACS Nano   Impact Factor & Quartile

1936-0851

1936-086X

DNA as an exceptional data storage medium offers high information density. However, DNA storage requires specialized equip-ment and tightly controlled environments for storage. Fast encapsulation within minutes for enhanced DNA stability to do away with specialized equipment and fast DNA extraction remain a challenge. Here, we report a DNA microlibrary that can be encapsulated by metal-organic frameworks (MOFs) within 10 min and extracted (5 min) in a single microfluidic chip for automated and integrated DNA-based data storage. The DNA microlibrary@MOFs enhances the stability of data-encoded DNA against harsh environments. The encoded information can be read out perfectly after accelerated aging, equivalent to being readable after 10 years of storage at 25 degrees C, 50% relative humidity, and 10 000 lx sunlight radiation. Moreover, the library enables fast retrieval of target data via flow cytometry and can be reproduced after each access.

metal &minus organic frameworks microfluidics encapsulation extraction DNA storage system reproducibility

SCI

English

NI Journal Papers

First ; Corresponding

National Key R&D Program of China["2021YFF1200100","2018YFA-0902600","2020YFA0908900"] ; National Natural Science Foundation of China["T2188102","22234004","21907032"] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000933947100001

AMER CHEMICAL SOC

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, Shenzhen Key Lab Smart Healthcare Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Dept Elect & Elect Engn, Shenzhen 518055, Guangdong, Peoples R China
3.Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China

Mao, Cuiping,Wang, Shuchen,Li, Jiankai,et al. Metal-Organic Frameworks in Microfluidics Enable Fast Encapsulation/Extraction of DNA for Automated and Integrated Data Storage[J]. ACS Nano,2023.

Mao, Cuiping.,Wang, Shuchen.,Li, Jiankai.,Feng, Zhuowei.,Zhang, Tong.,...&Jiang, Xingyu.(2023).Metal-Organic Frameworks in Microfluidics Enable Fast Encapsulation/Extraction of DNA for Automated and Integrated Data Storage.ACS Nano.

Mao, Cuiping,et al."Metal-Organic Frameworks in Microfluidics Enable Fast Encapsulation/Extraction of DNA for Automated and Integrated Data Storage".ACS Nano (2023).