Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction

Liu, Chuanfa1,2; Hao, Dongdong1,2; Sun, Ruixue1,2; Zhang, Yi1,2; Peng, Yang1,2; Yuan, Yang3,4; Jiang, Kai1,2,5; Li, Wenyang1,2; Wen, Xing1,2; Guo, Hongwei1,2

2023-02-01

10.1111/nph.18752

NEW PHYTOLOGIST   Impact Factor & Quartile

0028-646X

1469-8137

Metabolites including antibiotics, enzymes, and volatiles produced by plant-associated bacteria are key factors in plant-microbiota interaction that regulates various plant biological processes. There should be crucial mediators responsible for their entry into host plants. However, less is known about the identities of these plant transporters.We report that the Arabidopsis Nitrate Transporter1 (NRT1)/NPF protein NPF2.13 functions in plant uptake of tunicamycin (TM), a natural antibiotic produced by several Streptomyces spp., which inhibits protein N-glycosylation. Loss of NPF2.13 function resulted in enhanced TM tolerance, whereas NPF2.13 overexpression led to TM hypersensitivity. Transport assays confirmed that NPF2.13 is a H+/TM symporter and the transport is not affected by other substrates like nitrate. NPF2.13 exclusively showed TM transport activity among tested NPFs.Tunicamycin uptake from TM-producing Streptomyces upregulated the expression of nitrate-related genes including NPF2.13. Moreover, nitrate allocation to younger leaves was promoted by TM in host plants. Tunicamycin could also benefit plant defense against the pathogen. Notably, the TM effects were significantly repressed in npf2.13 mutant.Overall, this study identifies NPF2.13 protein as an important TM transporter in plant-microbe interaction and provides insights into multiple facets of NPF proteins in modulating plant nutrition and defense by transporting exterior bacterial metabolites.

Arabidopsis thaliana bacterial metabolites NPF2 13 plant-microbe interaction Streptomyces sp transport tunicamycin

SCI

English

First ; Corresponding

Shenzhen Science and Technology Innovation Commission[JCYJ20170817105725365] ; Shenzhen Science and Technology Program[KQTD20190929173906742] ; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes (SUSTech)[2019KSYS006] ; China Postdoctoral Science Foundation[2018M642898]

Plant Sciences

Plant Sciences

WOS:000929417800001

WILEY

PLANT & ANIMAL SCIENCE

Web of Science

1.Southern Univ Sci & Technol SUSTech, Inst Plant & Food Sci, Sch Life Sci, Dept Biol, Shenzhen 518055, Peoples R China
2.SUSTech, Key Lab Mol Design Plant Cell Factory, Guangdong Higher Educ Inst, Shenzhen 518055, Peoples R China
3.Nanjing Agr Univ, Crop Genom & Bioinformat Ctr, Appl Plant Genom Lab, Nanjing 210095, Jiangsu, Peoples R China
4.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
5.SUSTech, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China

Liu, Chuanfa,Hao, Dongdong,Sun, Ruixue,et al. Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction[J]. NEW PHYTOLOGIST,2023.

Liu, Chuanfa.,Hao, Dongdong.,Sun, Ruixue.,Zhang, Yi.,Peng, Yang.,...&Guo, Hongwei.(2023).Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction.NEW PHYTOLOGIST.

Liu, Chuanfa,et al."Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction".NEW PHYTOLOGIST (2023).