中文版 | English
Title

Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction

Author
Corresponding AuthorWen, Xing; Guo, Hongwei
Publication Years
2023-02-01
DOI
Source Title
ISSN
0028-646X
EISSN
1469-8137
Abstract
Metabolites including antibiotics, enzymes, and volatiles produced by plant-associated bacteria are key factors in plant-microbiota interaction that regulates various plant biological processes. There should be crucial mediators responsible for their entry into host plants. However, less is known about the identities of these plant transporters.We report that the Arabidopsis Nitrate Transporter1 (NRT1)/NPF protein NPF2.13 functions in plant uptake of tunicamycin (TM), a natural antibiotic produced by several Streptomyces spp., which inhibits protein N-glycosylation. Loss of NPF2.13 function resulted in enhanced TM tolerance, whereas NPF2.13 overexpression led to TM hypersensitivity. Transport assays confirmed that NPF2.13 is a H+/TM symporter and the transport is not affected by other substrates like nitrate. NPF2.13 exclusively showed TM transport activity among tested NPFs.Tunicamycin uptake from TM-producing Streptomyces upregulated the expression of nitrate-related genes including NPF2.13. Moreover, nitrate allocation to younger leaves was promoted by TM in host plants. Tunicamycin could also benefit plant defense against the pathogen. Notably, the TM effects were significantly repressed in npf2.13 mutant.Overall, this study identifies NPF2.13 protein as an important TM transporter in plant-microbe interaction and provides insights into multiple facets of NPF proteins in modulating plant nutrition and defense by transporting exterior bacterial metabolites.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
Funding Project
Shenzhen Science and Technology Innovation Commission[JCYJ20170817105725365] ; Shenzhen Science and Technology Program[KQTD20190929173906742] ; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes (SUSTech)[2019KSYS006] ; China Postdoctoral Science Foundation[2018M642898]
WOS Research Area
Plant Sciences
WOS Subject
Plant Sciences
WOS Accession No
WOS:000929417800001
Publisher
ESI Research Field
PLANT & ANIMAL SCIENCE
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/490023
DepartmentSUSTech-Peking University National Institute of Plants and Food
生命科学学院
前沿与交叉科学研究院
生命科学学院_生物系
Affiliation
1.Southern Univ Sci & Technol SUSTech, Inst Plant & Food Sci, Sch Life Sci, Dept Biol, Shenzhen 518055, Peoples R China
2.SUSTech, Key Lab Mol Design Plant Cell Factory, Guangdong Higher Educ Inst, Shenzhen 518055, Peoples R China
3.Nanjing Agr Univ, Crop Genom & Bioinformat Ctr, Appl Plant Genom Lab, Nanjing 210095, Jiangsu, Peoples R China
4.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
5.SUSTech, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China
First Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Corresponding Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
First Author's First AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Recommended Citation
GB/T 7714
Liu, Chuanfa,Hao, Dongdong,Sun, Ruixue,et al. Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction[J]. NEW PHYTOLOGIST,2023.
APA
Liu, Chuanfa.,Hao, Dongdong.,Sun, Ruixue.,Zhang, Yi.,Peng, Yang.,...&Guo, Hongwei.(2023).Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction.NEW PHYTOLOGIST.
MLA
Liu, Chuanfa,et al."Arabidopsis NPF2.13 functions as a critical transporter of bacterial natural compound tunicamycin in plant-microbe interaction".NEW PHYTOLOGIST (2023).
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