中文版 | English
Title

Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis

Author
Corresponding AuthorLiu,Xuncheng
Publication Years
2023-05-01
DOI
Source Title
ISSN
0168-9452
EISSN
1873-2259
Volume330
Abstract

Chlorophylls are the major pigments that harvest light energy during photosynthesis in plants. Although reactions in chlorophyll biogenesis have been largely known, little attention has been paid to the post-translational regulation mechanism of this process. In this study, we found that four lysine sites (K128/340/350/390) of NADPH:protochlorophyllide oxidoreductase A (PORA), which catalyzes the only light-triggered step in chlorophyll biosynthesis, were acetylated after dark-grown seedlings transferred to light via acetylomics analysis. Etiolated seedlings with K390 mutation of PORA had a lower greening rate and decreased PORA acetylation after illumination. Importantly, K390 of PORA was found extremely conserved in plants and cyanobacteria via bioinformatics analysis. We further demonstrated that the acetylation level of PORA was increased by exposing the dark-grown seedlings to the histone deacetylase (HDAC) inhibitor TSA. Thus, the HDACs probably regulate the acetylation of PORA, thereby controlling this non-histone substrate to catalyze the reduction of Pchlide to produce chlorophyllide, which provides a novel regulatory mechanism by which the plant actively tunes chlorophyll biosynthesis during the conversion from skotomorphogenesis to photomorphogenesis.

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
Funding Project
Guangdong Basic and Applied Basic Research Foundation Committee[2020A1515110711] ; Key Lab of Tropical and Subtropical Plant Resources of Guangdong Province[2019PlantKF08] ; National Natural Science Foundation of China["32070551","32101342"] ; Special Research Assistant Funding Project of the Chinese Academy of Sciences[2021000065] ; China Postdoctoral Science Foundation["2021M693220","2022T150656"]
WOS Research Area
Biochemistry & Molecular Biology ; Plant Sciences
WOS Subject
Biochemistry & Molecular Biology ; Plant Sciences
WOS Accession No
WOS:000954899100001
Publisher
ESI Research Field
PLANT & ANIMAL SCIENCE
Scopus EID
2-s2.0-85148762174
Data Source
Scopus
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/497229
DepartmentSUSTech-Peking University National Institute of Plants and Food
生命科学学院
生命科学学院_生物系
Affiliation
1.Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,510650,China
2.Guangdong Provincial Key Laboratory of Applied Botany,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,510650,China
3.Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,510650,China
4.College of Life Sciences,University of Chinese Academy of Sciences,Beijing,100049,China
5.College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,510630,China
6.Department of Biology,Institute of Plant and Food Science,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
Recommended Citation
GB/T 7714
Liang,Minting,Gu,Dachuan,Lie,Zhiyang,et al. Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis[J]. PLANT SCIENCE,2023,330.
APA
Liang,Minting.,Gu,Dachuan.,Lie,Zhiyang.,Yang,Yongyi.,Lu,Longxin.,...&Liu,Xuncheng.(2023).Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis.PLANT SCIENCE,330.
MLA
Liang,Minting,et al."Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis".PLANT SCIENCE 330(2023).
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