Title | Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis |
Author | |
Corresponding Author | Liu,Xuncheng |
Publication Years | 2023-05-01
|
DOI | |
Source Title | |
ISSN | 0168-9452
|
EISSN | 1873-2259
|
Volume | 330 |
Abstract | Chlorophylls are the major pigments that harvest light energy during photosynthesis in plants. Although reactions in chlorophyll biogenesis have been largely known, little attention has been paid to the post-translational regulation mechanism of this process. In this study, we found that four lysine sites (K128/340/350/390) of NADPH:protochlorophyllide oxidoreductase A (PORA), which catalyzes the only light-triggered step in chlorophyll biosynthesis, were acetylated after dark-grown seedlings transferred to light via acetylomics analysis. Etiolated seedlings with K390 mutation of PORA had a lower greening rate and decreased PORA acetylation after illumination. Importantly, K390 of PORA was found extremely conserved in plants and cyanobacteria via bioinformatics analysis. We further demonstrated that the acetylation level of PORA was increased by exposing the dark-grown seedlings to the histone deacetylase (HDAC) inhibitor TSA. Thus, the HDACs probably regulate the acetylation of PORA, thereby controlling this non-histone substrate to catalyze the reduction of Pchlide to produce chlorophyllide, which provides a novel regulatory mechanism by which the plant actively tunes chlorophyll biosynthesis during the conversion from skotomorphogenesis to photomorphogenesis. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
|
SUSTech Authorship | Others
|
Funding Project | Guangdong Basic and Applied Basic Research Foundation Committee[2020A1515110711]
; Key Lab of Tropical and Subtropical Plant Resources of Guangdong Province[2019PlantKF08]
; National Natural Science Foundation of China["32070551","32101342"]
; Special Research Assistant Funding Project of the Chinese Academy of Sciences[2021000065]
; China Postdoctoral Science Foundation["2021M693220","2022T150656"]
|
WOS Research Area | Biochemistry & Molecular Biology
; Plant Sciences
|
WOS Subject | Biochemistry & Molecular Biology
; Plant Sciences
|
WOS Accession No | WOS:000954899100001
|
Publisher | |
ESI Research Field | PLANT & ANIMAL SCIENCE
|
Scopus EID | 2-s2.0-85148762174
|
Data Source | Scopus
|
Citation statistics |
Cited Times [WOS]:0
|
Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/497229 |
Department | SUSTech-Peking University National Institute of Plants and Food 生命科学学院 生命科学学院_生物系 |
Affiliation | 1.Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,510650,China 2.Guangdong Provincial Key Laboratory of Applied Botany,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,510650,China 3.Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,510650,China 4.College of Life Sciences,University of Chinese Academy of Sciences,Beijing,100049,China 5.College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,510630,China 6.Department of Biology,Institute of Plant and Food Science,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China |
Recommended Citation GB/T 7714 |
Liang,Minting,Gu,Dachuan,Lie,Zhiyang,et al. Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis[J]. PLANT SCIENCE,2023,330.
|
APA |
Liang,Minting.,Gu,Dachuan.,Lie,Zhiyang.,Yang,Yongyi.,Lu,Longxin.,...&Liu,Xuncheng.(2023).Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis.PLANT SCIENCE,330.
|
MLA |
Liang,Minting,et al."Regulation of chlorophyll biosynthesis by light-dependent acetylation of NADPH:protochlorophyll oxidoreductase A in Arabidopsis".PLANT SCIENCE 330(2023).
|
Files in This Item: | There are no files associated with this item. |
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment