Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection
|Corresponding Author||Zhang，Yu Zhong; Shen，Qing Tao|
|Joint first author||Sun，Meiling; Liu，Mingdong|
White spot syndrome virus (WSSV) is one of the largest DNA viruses and the major pathogen responsible for white spot syndrome in crustaceans. The WSSV capsid is critical for genome encapsulation and ejection and exhibits the rod-shaped and oval-shaped structures during the viral life cycle. However, the detailed architecture of the capsid and the structural transition mechanism remain unclear. Here, using cryo-electron microscopy (cryo-EM), we obtained a cryo-EM model of the rod-shaped WSSV capsid and were able to characterize its ring-stacked assembly mechanism. Furthermore, we identified an oval-shaped WSSV capsid from intact WSSV virions and analyzed the structural transition mechanism from the oval-shaped to rod-shaped capsids induced by high salinity. These transitions, which decrease internal capsid pressure, always accompany DNA release and mostly eliminate the infection of the host cells. Our results demonstrate an unusual assembly mechanism of the WSSV capsid and offer structural insights into the pressure-driven genome release.
NI Journal Papers
National Key Research and Development Program of China[
|WOS Research Area|
Science & Technology - Other Topics
|WOS Accession No|
Web of Science
Cited Times [WOS]:1
|Document Type||Journal Article|
|Department||Department of Biology|
1.Frontiers Science Center for Deep Ocean Multispheres and Earth System & College of Marine Life Sciences,Ocean University of China,Qingdao,266003,China
2.School of Life Science,Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China
3.Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,266237,China
4.iHuman Institute and School of Life Science and Technology,ShanghaiTech University,201210,China
5.State Key Laboratory of Microbial Technology,Marine Biotechnology Research Center,Shandong University,Qingdao,266237,China
6.Key Laboratory of Marine Genetics and Breeding,Ministry of Education,College of Marine Life Sciences,Ocean University of China,Qingdao,266003,China
7.Institute of Evolution and Marine Biodiversity,Ocean University of China,Qingdao,266003,China
8.High Performance Computing Center,Shanghai Jiao Tong University,200240,China
|Corresponding Author Affilication||Department of Biology; School of Life Sciences|
Sun，Meiling,Liu，Mingdong,Shan，Hong,et al. Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection[J]. Science advances,2023,9(8).
Sun，Meiling.,Liu，Mingdong.,Shan，Hong.,Li，Kang.,Wang，Peng.,...&Shen，Qing Tao.(2023).Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection.Science advances,9(8).
Sun，Meiling,et al."Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection".Science advances 9.8(2023).
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