中文版 | English
Title

The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization

Author
Corresponding AuthorLiu, Bin
Publication Years
2023-01-10
DOI
Source Title
ISSN
1945-0877
EISSN
1937-9145
Volume16Issue:767
Abstract
Escherichia coli are part of the normal intestinal microbiome, but some enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) strains can cause potentially life-threatening gastroenteritis. Virulence factors underlying the ability of EHEC and EPEC to cause disease include those encoded in the locus of the enterocyte effacement (LEE) pathogenicity island. Here, we demonstrated that EsrL, a small RNA present in many E. coli strains, promoted pathogenicity, adhesion, and biofilm formation in EHEC and EPEC. PhoB, the response regu-lator of the two-component system that controls cellular responses to phosphate, directly repressed esrL expres-sion under low-phosphate conditions. A phosphate-rich environment, similar to that of the human intestine, relieved PhoB-mediated repression of esrL. EsrL interacted with and stabilized the LEE-encoded regulator (ler) transcript, which encodes a transcription factor for LEE genes, leading to increased bacterial adhesion to cul-tured cells and colonization of the rabbit colon. EsrL also bound to and stabilized the fimC transcript, which encodes a chaperone that is required for the assembly of type 1 pili, resulting in enhanced cell adhesion in pathogenic E. coli and enhanced biofilm formation in pathogenic and nonpathogenic E. coli. Our findings dem-onstrate that EsrL stimulates the expression of virulence genes in both EHEC and EPEC under phosphate-rich conditions, thus promoting the pathogenicity of EHEC and EPEC in the nutrient-rich gut environment.
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Others
Funding Project
National Natural Science Foundation of China Program["32070130","81772148","31820103002","31770144","81871624"] ; Distinguished Young Scholars of Tianjin[20JCJQJC00180] ; Natural Science Foundation (Key Project) of Tianjin[20JCZDJC00820] ; Natural Science Foundation of Shenzhen[JCYJ20220530164604010]
WOS Research Area
Biochemistry & Molecular Biology ; Cell Biology
WOS Subject
Biochemistry & Molecular Biology ; Cell Biology
WOS Accession No
WOS:000933631100001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/501432
DepartmentSchool of Medicine
Affiliation
1.Nankai Univ, Inst Translat Med Res, TEDA Inst Biol Sci & Biotechnol, Tianjin Union Med Ctr, Tianjin, Peoples R China
2.Nankai Univ, Key Lab Mol Microbiol & Technol, Minist Educ, Tianjin, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China
4.Minist Educ, Ctr Microbial Funct Ge n & Detect Technol, Tianjin, Peoples R China
5.Nankai Int Adv Res Inst, Shenzhen, Peoples R China
First Author AffilicationSchool of Medicine
Recommended Citation
GB/T 7714
Jia, Tianyuan,Wu, Pan,Liu, Bin,et al. The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization[J]. SCIENCE SIGNALING,2023,16(767).
APA
Jia, Tianyuan.,Wu, Pan.,Liu, Bin.,Liu, Miaomiao.,Mu, Huiqian.,...&Yang, Liang.(2023).The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization.SCIENCE SIGNALING,16(767).
MLA
Jia, Tianyuan,et al."The phosphate-induced small RNA EsrL promotes E. coli virulence, biofilm formation, and intestinal colonization".SCIENCE SIGNALING 16.767(2023).
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