Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis

Chen, Jianhai1,2,3; Zeng, Silue1,4; Xue, Qiang1,5; Hong, Yuchuan6; Liu, Liangjian1; Song, Liang1; Fang, Chihua4; Zhang, Hai5; Wang, Benguo2; Sedgwick, Adam C.7; Zhang, Peng3; Sessler, Jonathan L.8; Liu, Chengbo1; Chen, Jingqin1

2022-10-19

10.1073/pnas.2213373119/-/DCSupplemental

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Impact Factor & Quartile

0027-8424

1091-6490

The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead to improvements in the diagnosis and treatment of RA. Here, we report the combined use of small interfering RNAs (siRNAsT/I) and Prussian blue nanoparticles (PBNPs) to silence the expression of proinflammatory cytokines TNFa/IL-6 and scavenge the ROS associated with RAM. To enhance the in vitro and in vivo biological stability, biocompatibility, and targeting capability of the siRNAsT/I and PBNPs, macrophage membrane vesicles were used to prepare biomimetic nanoparticles, M@PsiRNAsT/I. The resulting constructs were found to suppress tumor necrosis factor-a/ interleukin-6 expression and overcome the hypoxic nature of RAM, thus alleviating RA-induced joint damage in a mouse model. The M@P-siRNAsT/I of this study could be monitored via near-infrared photoacoustic (PA) imaging. Moreover, multispectral PA imaging without the need for labeling permitted the real-time evaluation of M@PsiRNAsT/I as a putative RA treatment. Clinical microcomputed tomography and histological analysis confirmed the effectiveness of the treatment. We thus suggest that macrophage-biomimetic M@P-siRNAsT/I and their analogs assisted by PA imaging could provide a new strategy for RA diagnosis, treatment, and monitoring.

biomimetic nanoparticles photoacoustic rheumatoid arthritis targeted imaging small interfering RNA

SCI

English

NI Journal Papers

Others

National Natural Science Foundation of China[82172008] ; National Key R&D Program of China[2020YFA0908800] ; CAS Key Laboratory of Health Informatics[2011DP173015] ; Guangdong Provincial Key Laboratory of Biomedical Optical Imaging[2020B121201010] ; Guangdong Basic and Applied Basic Research Foundation[2020A1515010978] ; Robert A. Welch Foundation[F-0018]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000934528000002

NATL ACAD SCIENCES

BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING

Web of Science

1.Chinese Acad Sci, Shenzhen Inst Adv Technol, Res Lab Biomed Opt & Mol Imaging, CAS Key Lab Hlth Informat, Shenzhen 518055, Peoples R China
2.Chinese Univ Hong Kong, Longgang Dist Peoples Hosp Shenzhen, Affiliated Hosp 2, Rehabil Dept, Shenzhen 518172, Peoples R China
3.Chinese Acad Sci, Ctr Translat Med Res & Dev, Shenzhen Engn Res Ctr Med Bioact Mat, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
4.Southern Med Univ, Zhujiang Hosp, Dept Hepatobiliary Surg 1, Guangzhou 510280, Peoples R China
5.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Ultrasound,Affiliated Hosp 1,Clin Coll 2, Shenzhen 518020, Peoples R China
6.Chinese Acad Sci, Brain Cognit & Brain Dis Inst, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
7.Univ Oxford, Chem Res Lab, Oxford OX1 3TA, England
8.Univ Texas Austin, Dept Chem, Austin, TX 78712 USA

Chen, Jianhai,Zeng, Silue,Xue, Qiang,et al. Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(43).

Chen, Jianhai.,Zeng, Silue.,Xue, Qiang.,Hong, Yuchuan.,Liu, Liangjian.,...&Chen, Jingqin.(2022).Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(43).

Chen, Jianhai,et al."Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.43(2022).