中文版 | English
Title

An "AND" Molecular Logic Gate as a Super-Enhancers for De Novo Designing Activatable Probe and Its Application in Atherosclerosis Imaging

Author
Corresponding AuthorYu, Xiu; Dai, Cuilian; Zheng, Jinrong
Publication Years
2023-02-01
DOI
Source Title
EISSN
2198-3844
Abstract
Developing activatable fluorescent probes with superlative fluorescence enhancement factor (F/F-0) to improve the signal-to-noise (S/N) ratio is still an urgent issue. "AND" molecular logic gates are emerging as a useful tool for enhanced probes selectivity and accuracy. Here, an "AND" logic gate is developed as super-enhancers for designing activatable probes with huge F/F-0 and S/N ratio. It utilizes lipid-droplets (LDs) as controllable background input and sets the target analyte as variable input. The fluorescence is tremendously quenching due to double locking, thus an extreme F/F-0 ratio of target analyte is obtained. Importantly, this probe can transfer to LDs after a response occurs. The target analyte can be directly visualized through the spatial location without a control group. Accordingly, a peroxynitrite (ONOO-) activatable probe (CNP2-B) is de novo designed. The F/F-0 of CNP2-B achieves 2600 after reacting with ONOO-. Furthermore, CNP2-B can transfer from mitochondria to lipid droplets after being activated. The higher selectivity and S/N ratio of CNP2-B are obtained than commercial probe 3'-(p-hydroxyphenyl) fluorescein (HPFin vitro and in vivo. Therefore, the atherosclerotic plaques at mouse models are delineated clearly after administration with in situ CNP2-B probe gel. Such input controllable "AND" logic gate is envisioned to execute more imaging tasks.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Natural Science Foundation of China["32201145","82100443"] ; Xiamen Natural Science Foundation Youth Program[3502Z20227134]
WOS Research Area
Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS Subject
Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Accession No
WOS:000937666400001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/502117
DepartmentShenzhen People's Hospital
Affiliation
1.Xiamen Univ, Xiamen Cardiovasc Hosp, Inst Cardiovasc Dis, Sch Med, 2999 Jinshan Rd, Xiamen 361006, Peoples R China
2.Nanjing Univ Chinese Med, Nanjing Hosp Chinese Med, 157 Daming Rd, Nanjing 210000, Peoples R China
3.Gannan Med Univ, Sch Pharm, 1 Med Coll Rd, Ganzhou 341000, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Shenzhen Key Lab Resp Dis, 3046 Shennan East Rd, Shenzhen 518055, Peoples R China
Corresponding Author AffilicationShenzhen People's Hospital
Recommended Citation
GB/T 7714
Sang, Mangmang,Huang, Yibo,Wang, Lu,et al. An "AND" Molecular Logic Gate as a Super-Enhancers for De Novo Designing Activatable Probe and Its Application in Atherosclerosis Imaging[J]. ADVANCED SCIENCE,2023.
APA
Sang, Mangmang.,Huang, Yibo.,Wang, Lu.,Nawsherwan, Lei.,Chen, Lei.,...&Zheng, Jinrong.(2023).An "AND" Molecular Logic Gate as a Super-Enhancers for De Novo Designing Activatable Probe and Its Application in Atherosclerosis Imaging.ADVANCED SCIENCE.
MLA
Sang, Mangmang,et al."An "AND" Molecular Logic Gate as a Super-Enhancers for De Novo Designing Activatable Probe and Its Application in Atherosclerosis Imaging".ADVANCED SCIENCE (2023).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Export to Excel
Export to Csv
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Sang, Mangmang]'s Articles
[Huang, Yibo]'s Articles
[Wang, Lu]'s Articles
Baidu Scholar
Similar articles in Baidu Scholar
[Sang, Mangmang]'s Articles
[Huang, Yibo]'s Articles
[Wang, Lu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sang, Mangmang]'s Articles
[Huang, Yibo]'s Articles
[Wang, Lu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.