MXene-Based Dual Functional Nanocomposite with Photothermal Nanozyme Catalytic Activity to Fight Bacterial Infections

Yuan, Haitao4,8,9; Hong, Xiaoping1; Ma, Huan5; Fu, Chunjin8,9; Guan, Yudong8,9; Huang, Wei6; Ma, Jingbo8,9; Xia, Peng7; Cao, Min8,9; Zheng, Liuhai8,9; Xu, Xiaolong8,9; Xu, Chengchao2,3; Liu, Dongzhou1; Li, Zhijie8,9; Geng, Qingshan10; Wang, Jigang2,3,8,9

2023-02-01

10.1021/acsmaterialslett.2c00771

ACS MATERIALS LETTERS   Impact Factor & Quartile

2639-4979

Bacterial infections are common complications for diabetic wounds and represent a considerable challenge for wound therapy. Diabetic wound infections differ from those of normal wounds. Owing to the special microenvironment around them, diabetic wounds are more susceptible to infection and are difficult to heal. Nanozymes are of great significance to treat diabetic wound bacterial infections through unique catalytic activities, particularly for controlling drugresistant bacteria. However, their intrinsically low catalytic activity largely restricts their bactericidal function. Therefore, it is crucial to design and develop novel antibacterial modalities with multiple mechanisms of action. In this study, we design and synthesize a CeO2/Nb2C nanocomposite with dual functions of peroxidase activity and an excellent near-infrared (NIR) photothermal property. Under 808 nm laser irradiation, the CeO2/Nb2C nanocomposite produced a photothermal antibacterial effect and simultaneously displayed a synergistic enzyme catalytic property, thereby killing bacteria in a sustained manner with more than 80% sterilization ratio. The CeO2/Nb2C nanocomposite could accelerate the recovery of diabetic wounds when the skin lesions infected with methicillin-resistant Staphylococcus aureus (MRSA) were irradiated with an 808 nm laser in a diabetic mouse model. An RNA sequencing assay was used to profile the dynamic transcriptome of MRSA. The data set reveals that bacteria experience dysfunction in energy metabolism, cell morphology, and oxidative stress systems during the treatment with CeO2/ Nb2C nanocomposite under NIR irradiation, which further affects their survival. Moreover, the CeO2/Nb2C nanocomposite exhibits good biosafety in vitro and in vivo, which indicates their potency as antibacterial agents. This study provides a novel antibacterial strategy by the combination of the catalytic sterilization with the NIR photothermal activity of nanozymes for the effective treatment of MRSA-related diabetic wound infection.

SCI

English

Corresponding

Shenzhen Governmental Sustainable Development Fund["2020YFA0908000","2022YFC2303600"] ; Shenzhen key Laboratory of Kidney Diseases[ZYYCXTD-C-202002] ; Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties["CI2021A05101","CI2021A05104"] ; Science and Technology Foundation of Shenzhen["82141001","82274182","82074098","82173914"] ; National Key R&D Program of China Key projects for international cooperation on science, technology and innovation[CI2021B014] ; High-level Hospital Construction Project of Guangdong Provincial People's Hospital["ZZ14-YQ-050","ZZ14-YQ-051","ZZ14-YQ-052","ZZ14-FL-002","ZZ14-ND-010","ZZ15-ND-10"] ; Basic and Applied Basic Research Foundation of Guang Dong Province[KCXFZ20201221173612034] ; International Science and Technology Cooperation for Shenzhen Technology Innovation Plan[ZDSYS201504301616234] ; Chinese Postdoctoral Science Foundation[SZGSP001] ; null[JCYJ20210324115800001] ; null[JCYJ20210324113608023] ; null[2020YFE0205100] ; null[DFJH201811] ; null[DFJH201922] ; null[2021A1515012164] ; null[GJHZ20200731095411034] ; null[2022M712190]

Materials Science

Materials Science, Multidisciplinary

WOS:000940341800001

AMER CHEMICAL SOC

Web of Science

1.Jinan Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp, Dept Rheumatol & Immunol,Clin Med Coll 2,Southern, Shenzhen 518020, Guangdong, Peoples R China
2.China Acad Chinese Med Sci, Artemisinin Res Ctr, Beijing 100700, Peoples R China
3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
4.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou 510632, Peoples R China
5.Guangdong Prov Peoples Hosp, Guangdong Cardiovasc Inst, Guangdong Acad Med Sci, Guangzhou 510050, Guangdong, Peoples R China
6.Sun Yat Sen Univ, Sch Chem Engn & Technol, Zhuhai 519082, Peoples R China
7.Wuhan Univ, Dept Hepatobiliary & Pancreat Surg, Zhongnan Hosp, Wuhan 430062, Peoples R China
8.Jinan Univ, Dept Nephrol, Shenzhen Key Lab Kidney Dis, Shenzhen 518020, Peoples R China
9.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2, Shenzhen 518020, Peoples R China
10.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Shenzhen Clin Res Ctr Geriatr,Cl, Shenzhen 518020, Peoples R China

Yuan, Haitao,Hong, Xiaoping,Ma, Huan,et al. MXene-Based Dual Functional Nanocomposite with Photothermal Nanozyme Catalytic Activity to Fight Bacterial Infections[J]. ACS MATERIALS LETTERS,2023.

Yuan, Haitao.,Hong, Xiaoping.,Ma, Huan.,Fu, Chunjin.,Guan, Yudong.,...&Wang, Jigang.(2023).MXene-Based Dual Functional Nanocomposite with Photothermal Nanozyme Catalytic Activity to Fight Bacterial Infections.ACS MATERIALS LETTERS.

Yuan, Haitao,et al."MXene-Based Dual Functional Nanocomposite with Photothermal Nanozyme Catalytic Activity to Fight Bacterial Infections".ACS MATERIALS LETTERS (2023).