A nanoparticle-coated microfluidic chip for automated, non-destructive extraction of encapsulated DNA in data storage

Geng, Chunyang1,2; Liu, Shaoqin1; Jiang, Xingyu1,2

2023-02-01

10.1039/d2sc06466a

Chemical Science   Impact Factor & Quartile

2041-6520

2041-6539

DNA data storage based on tubes as physical storage carriers has been developed to solve the problem of the exponential growth of information. Compact disk (CD)-microfluidics that employs centrifugal forces for fluidic manipulation offers an attractive alternative that integrates complex assays onto a miniaturized platform to result in automation for DNA data storage. In this work, we develop a CD microfluidic chip modified with nanoparticles for accurate fluid flow control. The nanoparticle coating turns microchannels into valves or pumps, which reduces the error in fluidic control from 62% to 6%. Based on the nanoparticle coating, the chip integrates demineralization, nucleic acid amplification, and re-mineralization functions for automated, non-destructive information extraction. We prove the functionality of the chip with mineralized DNA data. Compared to purely manual operation and traditional amplification techniques, the microfluidic chip saves human power and time consumption, demonstrating an essential contribution to the development of DNA data storage.

SCI

English

NI Journal Papers

Corresponding

National Key R&D Program of China["2018YFA0902600","2021YFF1200801","2021YFF1200802","2020YFA0908900","2021YFF1200103"] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Shenzhen Science and Technology Program["JCYJ20200109141231365","21535001"] ; National Natural Science Foundation of China["81730051","32071390","QYZDJ-SSW-SLH039"] ; Chinese Academy of Sciences["121D11KYSB20170026","ZDSYS20200811144003009"] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[2019ZT08Y191] ; Guangdong Innovative and Entrepreneurial Research Team Program[SZBL2019062801004] ; null[KQTD20190929172743294]

Chemistry

Chemistry, Multidisciplinary

WOS:000941196100001

ROYAL SOC CHEMISTRY

Web of Science

1.Harbin Inst Technol, Sch Life Sci & Technol, 2 Yikuang Rd, Harbin 150001, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, Guangdong Prov Key Lab Adv Biomat, 1088,Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China

Geng, Chunyang,Liu, Shaoqin,Jiang, Xingyu. A nanoparticle-coated microfluidic chip for automated, non-destructive extraction of encapsulated DNA in data storage[J]. Chemical Science,2023.

Geng, Chunyang,Liu, Shaoqin,&Jiang, Xingyu.(2023).A nanoparticle-coated microfluidic chip for automated, non-destructive extraction of encapsulated DNA in data storage.Chemical Science.

Geng, Chunyang,et al."A nanoparticle-coated microfluidic chip for automated, non-destructive extraction of encapsulated DNA in data storage".Chemical Science (2023).