Perfluorononanoic Acid Induces Neurotoxicity via Synaptogenesis Signaling in Zebrafish

Liu, Shuai1; Qiu, Wenhui2; Li, Rongzhen2; Chen, Bei3; Wu, Xin2; Magnuson, Jason T.4; Xu, Bentuo5; Luo, Shusheng2; Xu, Elvis Genbo6; Zheng, Chunmiao2

2023-02-01

10.1021/acs.est.2c06739

ENVIRONMENTAL SCIENCE & TECHNOLOGY   Impact Factor & Quartile

0013-936X

1520-5851

Perfluorononanoic acid (PFNA), commonly used as an alternative polyfluorinated compound (PFC) of perfluorooctanoic acid (PFOA), has been widely detected in the aquatic environment. Previous ecotoxicological and epidemiological results suggested that some neurobehavioral effects were associated with PFC exposure; however, the ecological impacts and underlying neurotoxicity mechanisms remain unclear, particularly in aquatic organisms during sensitive, early developmental stages. In this study, zebrafish embryos were exposed to environmentally relevant concentrations of PFNA for 120 h, and the neurological effects of PFNA were comprehensively assessed using transcriptional, biochemical, morphological, and behavioral assays. RNA sequencing and advanced bioinformatics analyses predicted and characterized the key biological processes and pathways affected by PFNA exposure, which included the synaptogenesis signaling pathway, neurotransmitter synapse, and CREB signaling in neurons. Neurotransmitter levels (acetylcholine, glutamate, 5-hydroxytryptamine, gamma-aminobutyric acid, dopamine, and noradrenaline) were significantly decreased in zebrafish larvae, and the Tg(gad67:GFP) transgenic line revealed a decreased number of GABAergic neurons in PFNA-treated larvae. Moreover, the swimming distance, rotation frequency, and activity degree were also significantly affected by PFNA, linking molecular-level changes to behavioral consequences.

perfluorononanoic acid embryo Danio rerio neurotransmitter early life stage behavior

SCI

English

NI Journal Papers

Corresponding

National Natural Science Foundation of China["42077223","42277266"] ; National Key Research and Development Program of China[2021YFA1202500] ; Shenzhen Science and Technology Innovation Committee["JCYJ20190809164201686","KQTD2016022619584022","KCXFZ20201221173410029"] ; State Environmental Pro-tection Key Laboratory of Integrated Surface Water-Ground-water Pollution Control, Guangdong Provincial Key Labo-ratory of Soil and Ground-water Pollution Control[2017B030301012]

Engineering ; Environmental Sciences & Ecology

Engineering, Environmental ; Environmental Sciences

WOS:000946540200001

AMER CHEMICAL SOC

ENVIRONMENT/ECOLOGY

Web of Science

1.Jiangxi Acad Sci, Inst Microbiol, Nanchang 330012, Peoples R China
2.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, Shenzhen 518055, Peoples R China
3.Fisheries Res Inst Fujian, Xiamen 361000, Peoples R China
4.Univ Stavanger, Dept Chem Biosci & Environm Engn, N-4021 Stavanger, Norway
5.Wenzhou Univ, Natl & Local Joint Engn Res Ctr Ecol Treatment Tec, Sch Life & Environm Sci, Wenzhou 325035, Peoples R China
6.Univ Southern Denmark, Dept Biol, DK-5230 Odense, Denmark

Liu, Shuai,Qiu, Wenhui,Li, Rongzhen,et al. Perfluorononanoic Acid Induces Neurotoxicity via Synaptogenesis Signaling in Zebrafish[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2023.

Liu, Shuai.,Qiu, Wenhui.,Li, Rongzhen.,Chen, Bei.,Wu, Xin.,...&Zheng, Chunmiao.(2023).Perfluorononanoic Acid Induces Neurotoxicity via Synaptogenesis Signaling in Zebrafish.ENVIRONMENTAL SCIENCE & TECHNOLOGY.

Liu, Shuai,et al."Perfluorononanoic Acid Induces Neurotoxicity via Synaptogenesis Signaling in Zebrafish".ENVIRONMENTAL SCIENCE & TECHNOLOGY (2023).