Title | Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs |
Author | |
Corresponding Author | Xia, Yu |
Joint first author | Zhao, Bixi; Chen, Liming |
Publication Years | 2023-03-14
|
DOI | |
Source Title | |
ISSN | 0013-936X
|
EISSN | 1520-5851
|
Volume | 57Issue:10 |
Abstract | Although conductive materials have been shown to improve efficiency in anaerobic digestion (AD) by modifying microbial interactions, the interacting network under thermophilic conditions has not been examined. To identify the true taxon-taxon associations within thermophilic anaerobic digestion (TAD) microbiome and reveal the influence of carbon cloth (CC) addition, we sampled micron-scale single flocs (40-70 mu m) randomly isolated from lab-scale thermophilic digesters. Results revealed that CC addition not only significantly boosted methane yield by 25.3% but also increased the spatial heterogeneity of the community in the sludge medium. After CC addition, an evident translocation of Pseudomonas from the medium to the biofilm was observed, showing their remarkable capacity for biofilm formation. Additionally, Clostridium and Thermotogaceae tightly aggregated and steadily co-occurred in the medium and biofilm of the TAD microbiome, which might be associated with their unique extracellular sugar metabolizing style. Finally, CC induced syntrophic interaction between Syntrophomonas and denitrifiers of Rhodocyclaceae. The upregulated respiration-associated electron transferring genes (Cyst-c, complex III) on the cellular membranes of these collaborating partners indicated a potential coupling of the denitrification pathway with syntrophic acetate oxidation via direct interspecies electron transfer (DIET). These findings provide an insight into how conductive materials promote thermophilic digestion performance and open the path for improved community monitoring of biotreatment systems. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
|
Important Publications | NI Journal Papers
|
SUSTech Authorship | First
; Corresponding
|
Funding Project | National Key Research and Development Program of China[2021YFA1202500]
; Shenzhen Science and Technology Innovation Committee[JCYJ20210324104412033]
; National Natural Science Foundation of China[52000099]
|
WOS Research Area | Engineering
; Environmental Sciences & Ecology
|
WOS Subject | Engineering, Environmental
; Environmental Sciences
|
WOS Accession No | WOS:000941546100001
|
Publisher | |
ESI Research Field | ENVIRONMENT/ECOLOGY
|
Data Source | Web of Science
|
Citation statistics |
Cited Times [WOS]:0
|
Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/513439 |
Department | College of Engineering 工学院_环境科学与工程学院 |
Affiliation | 1.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, Shenzhen, Peoples R China 2.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Shenzhen 518055, Peoples R China 3.Southern Univ Sci & Technol, Sch Environmenta lScience & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, Shenzhen 518055, Peoples R China 4.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, Shenzhen, Peoples R China 5.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Shenzhen 518055, Peoples R China 6.Southern Univ Sci & Technol, Sch Environmenta lScience & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, Shenzhen 518055, Peoples R China |
First Author Affilication | College of Engineering; School of Environmental Science and Engineering |
Corresponding Author Affilication | College of Engineering; School of Environmental Science and Engineering; Southern University of Science and Technology |
First Author's First Affilication | College of Engineering; School of Environmental Science and Engineering |
Recommended Citation GB/T 7714 |
Zhao, Bixi,Chen, Liming,Zhang, Miao,et al. Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2023,57(10).
|
APA |
Zhao, Bixi.,Chen, Liming.,Zhang, Miao.,Nie, Cailong.,Yang, Qing.,...&Xia, Yu.(2023).Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs.ENVIRONMENTAL SCIENCE & TECHNOLOGY,57(10).
|
MLA |
Zhao, Bixi,et al."Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs".ENVIRONMENTAL SCIENCE & TECHNOLOGY 57.10(2023).
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