中文版 | English
Title

Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs

Author
Corresponding AuthorXia, Yu
Joint first authorZhao, Bixi; Chen, Liming
Publication Years
2023-03-14
DOI
Source Title
ISSN
0013-936X
EISSN
1520-5851
Volume57Issue:10
Abstract

Although conductive materials have been shown to improve efficiency in anaerobic digestion (AD) by modifying microbial interactions, the interacting network under thermophilic conditions has not been examined. To identify the true taxon-taxon associations within thermophilic anaerobic digestion (TAD) microbiome and reveal the influence of carbon cloth (CC) addition, we sampled micron-scale single flocs (40-70 mu m) randomly isolated from lab-scale thermophilic digesters. Results revealed that CC addition not only significantly boosted methane yield by 25.3% but also increased the spatial heterogeneity of the community in the sludge medium. After CC addition, an evident translocation of Pseudomonas from the medium to the biofilm was observed, showing their remarkable capacity for biofilm formation. Additionally, Clostridium and Thermotogaceae tightly aggregated and steadily co-occurred in the medium and biofilm of the TAD microbiome, which might be associated with their unique extracellular sugar metabolizing style. Finally, CC induced syntrophic interaction between Syntrophomonas and denitrifiers of Rhodocyclaceae. The upregulated respiration-associated electron transferring genes (Cyst-c, complex III) on the cellular membranes of these collaborating partners indicated a potential coupling of the denitrification pathway with syntrophic acetate oxidation via direct interspecies electron transfer (DIET). These findings provide an insight into how conductive materials promote thermophilic digestion performance and open the path for improved community monitoring of biotreatment systems.

Keywords
URL[Source Record]
Indexed By
Language
English
Important Publications
NI Journal Papers
SUSTech Authorship
First ; Corresponding
Funding Project
National Key Research and Development Program of China[2021YFA1202500] ; Shenzhen Science and Technology Innovation Committee[JCYJ20210324104412033] ; National Natural Science Foundation of China[52000099]
WOS Research Area
Engineering ; Environmental Sciences & Ecology
WOS Subject
Engineering, Environmental ; Environmental Sciences
WOS Accession No
WOS:000941546100001
Publisher
ESI Research Field
ENVIRONMENT/ECOLOGY
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/513439
DepartmentCollege of Engineering
工学院_环境科学与工程学院
Affiliation
1.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Sch Environmenta lScience & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, Shenzhen 518055, Peoples R China
4.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, Shenzhen, Peoples R China
5.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Shenzhen 518055, Peoples R China
6.Southern Univ Sci & Technol, Sch Environmenta lScience & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, Shenzhen 518055, Peoples R China
First Author AffilicationCollege of Engineering;  School of Environmental Science and Engineering
Corresponding Author AffilicationCollege of Engineering;  School of Environmental Science and Engineering;  Southern University of Science and Technology
First Author's First AffilicationCollege of Engineering;  School of Environmental Science and Engineering
Recommended Citation
GB/T 7714
Zhao, Bixi,Chen, Liming,Zhang, Miao,et al. Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2023,57(10).
APA
Zhao, Bixi.,Chen, Liming.,Zhang, Miao.,Nie, Cailong.,Yang, Qing.,...&Xia, Yu.(2023).Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs.ENVIRONMENTAL SCIENCE & TECHNOLOGY,57(10).
MLA
Zhao, Bixi,et al."Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs".ENVIRONMENTAL SCIENCE & TECHNOLOGY 57.10(2023).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Export to Excel
Export to Csv
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[Zhao, Bixi]'s Articles
[Chen, Liming]'s Articles
[Zhang, Miao]'s Articles
Baidu Scholar
Similar articles in Baidu Scholar
[Zhao, Bixi]'s Articles
[Chen, Liming]'s Articles
[Zhang, Miao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Bixi]'s Articles
[Chen, Liming]'s Articles
[Zhang, Miao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.