Title | Delivery of spike-RBD by bacterial type three secretion system for SARS-CoV-2 vaccine development |
Author | |
Corresponding Author | Wu, Weihui; Li, Liang; Bai, Fang |
Publication Years | 2023-02-21
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DOI | |
Source Title | |
ISSN | 1664-3224
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Volume | 14 |
Abstract | COVID-19 pandemic continues to spread throughout the world with an urgent demand for a safe and protective vaccine to effectuate herd protection and control the spread of SARS-CoV-2. Here, we report the development of a bacterial vector COVID-19 vaccine (aPA-RBD) that carries the gene for the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. Live-attenuated strains of Pseudomonas aeruginosa (aPA) were constructed which express the recombinant RBD and effectively deliver RBD protein into various antigen presenting cells through bacterial type 3 secretion system (T3SS) in vitro. In mice, two-dose of intranasal aPA-RBD vaccinations elicited the development of RBD-specific serum IgG and IgM. Importantly, the sera from the immunized mice were able to neutralize host cell infections by SARS-CoV-2 pseudovirus as well as the authentic virus variants potently. T-cell responses of immunized mice were assessed by enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICS) assays. aPA-RBD vaccinations can elicit RBD-specific CD4(+)and CD8(+)T cell responses. T3SS-based RBD intracellular delivery heightens the efficiency of antigen presentation and enables the aPA-RBD vaccine to elicit CD8(+)T cell response. Thus, aPA vector has the potential as an inexpensive, readily manufactured, and respiratory tract vaccination route vaccine platform for other pathogens |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | Corresponding
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Funding Project | National Natural Science Foundation of China["31870130","31970680","31970179","32170199","82061148018"]
; National Key Research and Development Project of China["2021YFE0201300","2021YFE0101700"]
; National Research Foundation of Korea[NRF-2020K2A9A2A11102267]
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WOS Research Area | Immunology
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WOS Subject | Immunology
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WOS Accession No | WOS:000943461400001
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Publisher | |
Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:0
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Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/513449 |
Department | School of Medicine |
Affiliation | 1.Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Key Lab Mol Microbiol, Tianjin, Peoples R China 2.Shenzhen Ctr Dis Control & Prevent, Dept Pathogen Biol, Shenzhen, Peoples R China 3.Peking Univ, Shenzhen Hosp, Intervent & Cell Therapy Ctr, Shenzhen, Peoples R China 4.Peking Univ, Hong Kong Univ Sci, Technol Med Ctr, Shenzhen, Peoples R China 5.Southern Univ Sci & Technol, Sch Med, Dept Pharmacol, Shenzhen, Peoples R China 6.Korea Univ, Dept Biotechnol & Bioinformat, Sejong, South Korea |
Corresponding Author Affilication | School of Medicine |
Recommended Citation GB/T 7714 |
Zhou, Yuchen,Qu, Jing,Sun, Xiaomeng,et al. Delivery of spike-RBD by bacterial type three secretion system for SARS-CoV-2 vaccine development[J]. Frontiers in Immunology,2023,14.
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APA |
Zhou, Yuchen.,Qu, Jing.,Sun, Xiaomeng.,Yue, Zhuo.,Liu, Yingzi.,...&Bai, Fang.(2023).Delivery of spike-RBD by bacterial type three secretion system for SARS-CoV-2 vaccine development.Frontiers in Immunology,14.
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MLA |
Zhou, Yuchen,et al."Delivery of spike-RBD by bacterial type three secretion system for SARS-CoV-2 vaccine development".Frontiers in Immunology 14(2023).
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