中文版 | English
Title

On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies

Author
Corresponding AuthorZhou, Youping
Publication Years
2023-03-01
DOI
Source Title
ISSN
0003-2700
EISSN
1520-6882
Abstract
The 18O/16O ratio of alpha-cellulose in land plants has proved of interest for climate, environmental, physiological, and metabolic studies. Reliable application of such a ratio may be compromised by the presence of hemicellulose impurities in the alpha- cellulose product obtainable with current extraction methods, as the impurities are known to be isotopically different from that of the alpha-cellulose. We first compared the quality of hydrolysates of "alpha- cellulose products" obtained with four representative extraction methods (Jayme and Wise; Brendel; Zhou; Loader) and quantified the hemicellulose-derived non-glucose sugars in the alpha-cellulose products from 40 land grass species using gas chromatography- mass spectrometry (GC/MS). Second, we performed compound specific isotope analysis of the hydrolysates using GC/Pyrolysis/ IRMS. These results were then compared with the bulk isotope analysis using EA/Pyrolysis/IRMS of the alpha-cellulose products. We found that overall, the Zhou method afforded the highest purity alpha-cellulose as judged by the minimal presence of lignin and the second-lowest presence of non-glucose sugars. Isotopic analysis then showed that the O-2-O-6 of the alpha-cellulose glucosyl units were all depleted in 18O by 0.0-4.3 mUr (average, 1.9 mUr) in a species-dependent manner relative to the alpha-cellulose products. The positive isotopic bias of using the alpha-cellulose product instead of the glucosyl units stems mainly from the fact that the pentoses that dominate hemicellulose contamination in the alpha-cellulose product are relatively enriched in 18O (compared to hexoses) as they inherit only the relatively 18O-enriched O-2-O-5 moiety of sucrose, the common precursor of pentoses and hexoses in cellulose, and are further enriched in 18O by the (incomplete) hydrolysis.
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
Chinese MOST[2018YFE0206400] ; Chinese NSF["42250203","41973072","32071507","42007408"]
WOS Research Area
Chemistry
WOS Subject
Chemistry, Analytical
WOS Accession No
WOS:000946471800001
Publisher
ESI Research Field
CHEMISTRY
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/523931
DepartmentDepartment of Ocean Science and Engineering
Affiliation
1.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Isotop Chem Biol ICB, Xian 710021, Peoples R China
2.Southern Univ Sci & Technol, Dept Ocean Sci & Engn, Isotop Chem & Biol Oceanog ICBO, Shenzhen 518055, Peoples R China
3.James Cook Univ, Coll Sci & Engn, ARC Ctr Excellence Australian Biodivers & Heritage, Ctr Trop Environm & Sustainabil Sci, Cairns 4878, Australia
4.Australian Natl Univ, Res Sch Biol, Acton, ACT 2601, Australia
Corresponding Author AffilicationDepartment of Ocean Science and Engineering
Recommended Citation
GB/T 7714
Rani, Andleeb,Zhao, Yu,Yan, Qiulin,et al. On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies[J]. ANALYTICAL CHEMISTRY,2023.
APA
Rani, Andleeb.,Zhao, Yu.,Yan, Qiulin.,Wang, Ying.,Ma, Ran.,...&Zhou, Youping.(2023).On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies.ANALYTICAL CHEMISTRY.
MLA
Rani, Andleeb,et al."On the Chemical Purity and Oxygen Isotopic Composition of alpha-Cellulose Extractable from Higher Plants and the Implications for Climate, Metabolic, and Physiological Studies".ANALYTICAL CHEMISTRY (2023).
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