中文版 | English
Title

Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations

Author
Corresponding AuthorZeng, Fuxing
Joint first authorYu, Ting; Jiang, Junyi
Publication Years
2023-03-01
DOI
Source Title
EISSN
2218-273X
Volume13Issue:3
Abstract

Magnesium ions are abundant and play indispensable functions in the ribosome. A decrease in Mg2+ concentration causes 70S ribosome dissociation and subsequent unfolding. Structural distortion at low Mg2+ concentrations has been observed in an immature pre50S, while the structural changes in mature subunits have not yet been studied. Here, we purified the 30S subunits of E. coli cells under various Mg2+ concentrations and analyzed their structural distortion by cryo-electron microscopy. Upon systematically interrogating the structural heterogeneity within the 1 mM Mg2+ dataset, we observed 30S particles with different levels of structural distortion in the decoding center, h17, and the 30S head. Our model showed that, when the Mg2+ concentration decreases, the decoding center distorts, starting from h44 and followed by the shifting of h18 and h27, as well as the dissociation of ribosomal protein S12. Mg2+ deficiency also eliminates the interactions between h17, h10, h15, and S16, resulting in the movement of h17 towards the tip of h6. More flexible structures were observed in the 30S head and platform, showing high variability in these regions. In summary, the structures resolved here showed several prominent distortion events in the decoding center and h17. The requirement for Mg2+ in ribosomes suggests that the conformational changes reported here are likely shared due to a lack of cellular Mg2+ in all domains of life.

Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
First ; Corresponding
Funding Project
Guangdong Basic and Applied Basic Research Foundation[2021A1515010805] ; National Natural Science Foundation of China[32171200] ; Shenzhen Science and Technology Program[JCYJ20220530115210023]
WOS Research Area
Biochemistry & Molecular Biology
WOS Subject
Biochemistry & Molecular Biology
WOS Accession No
WOS:000957411000001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/523933
DepartmentSchool of Life Sciences
生命科学学院_系统生物学系
Affiliation
Southern Univ Sci & Technol, Sch Life Sci, Dept Syst Biol, 1088 Xueyuan Ave, Shenzhen 518055, Peoples R China
First Author AffilicationSchool of Life Sciences
Corresponding Author AffilicationSchool of Life Sciences
First Author's First AffilicationSchool of Life Sciences
Recommended Citation
GB/T 7714
Yu, Ting,Jiang, Junyi,Yu, Qianxi,et al. Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations[J]. BIOMOLECULES,2023,13(3).
APA
Yu, Ting,Jiang, Junyi,Yu, Qianxi,Li, Xin,&Zeng, Fuxing.(2023).Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations.BIOMOLECULES,13(3).
MLA
Yu, Ting,et al."Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations".BIOMOLECULES 13.3(2023).
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