Title | Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations |
Author | |
Corresponding Author | Zeng, Fuxing |
Joint first author | Yu, Ting; Jiang, Junyi |
Publication Years | 2023-03-01
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DOI | |
Source Title | |
EISSN | 2218-273X
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Volume | 13Issue:3 |
Abstract | Magnesium ions are abundant and play indispensable functions in the ribosome. A decrease in Mg2+ concentration causes 70S ribosome dissociation and subsequent unfolding. Structural distortion at low Mg2+ concentrations has been observed in an immature pre50S, while the structural changes in mature subunits have not yet been studied. Here, we purified the 30S subunits of E. coli cells under various Mg2+ concentrations and analyzed their structural distortion by cryo-electron microscopy. Upon systematically interrogating the structural heterogeneity within the 1 mM Mg2+ dataset, we observed 30S particles with different levels of structural distortion in the decoding center, h17, and the 30S head. Our model showed that, when the Mg2+ concentration decreases, the decoding center distorts, starting from h44 and followed by the shifting of h18 and h27, as well as the dissociation of ribosomal protein S12. Mg2+ deficiency also eliminates the interactions between h17, h10, h15, and S16, resulting in the movement of h17 towards the tip of h6. More flexible structures were observed in the 30S head and platform, showing high variability in these regions. In summary, the structures resolved here showed several prominent distortion events in the decoding center and h17. The requirement for Mg2+ in ribosomes suggests that the conformational changes reported here are likely shared due to a lack of cellular Mg2+ in all domains of life. |
Keywords | |
URL | [Source Record] |
Indexed By | |
Language | English
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SUSTech Authorship | First
; Corresponding
|
Funding Project | Guangdong Basic and Applied Basic Research Foundation[2021A1515010805]
; National Natural Science Foundation of China[32171200]
; Shenzhen Science and Technology Program[JCYJ20220530115210023]
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WOS Research Area | Biochemistry & Molecular Biology
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WOS Subject | Biochemistry & Molecular Biology
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WOS Accession No | WOS:000957411000001
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Publisher | |
Data Source | Web of Science
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Citation statistics |
Cited Times [WOS]:0
|
Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/523933 |
Department | School of Life Sciences 生命科学学院_系统生物学系 |
Affiliation | Southern Univ Sci & Technol, Sch Life Sci, Dept Syst Biol, 1088 Xueyuan Ave, Shenzhen 518055, Peoples R China |
First Author Affilication | School of Life Sciences |
Corresponding Author Affilication | School of Life Sciences |
First Author's First Affilication | School of Life Sciences |
Recommended Citation GB/T 7714 |
Yu, Ting,Jiang, Junyi,Yu, Qianxi,et al. Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations[J]. BIOMOLECULES,2023,13(3).
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APA |
Yu, Ting,Jiang, Junyi,Yu, Qianxi,Li, Xin,&Zeng, Fuxing.(2023).Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations.BIOMOLECULES,13(3).
|
MLA |
Yu, Ting,et al."Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations".BIOMOLECULES 13.3(2023).
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