Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-KB signaling pathways

Zhang, Shifen1,2,6,7; Lai, Qiaohua2,3; Liu, Liming1,2; Yang, Yajie4; Wang, Juan5

2022

10.15586/aei.v50i6.674

ALLERGOLOGIA ET IMMUNOPATHOLOGIA   Impact Factor & Quartile

0301-0546

1578-1267

["Objective: To investigate the effects of morroniside on inflammatory and oxidative stress in lipopolysaccharide (LPS)-induced inflammatory bowel disease (IBD) cell model.","Methods: NCM460 cells were treated with 2-, 5-, or 10-mu g/mL LPS for 24 h to develop an IBD cell model. MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) colorimet-ric assay was performed to uncover the role of morroniside on the viability of LPS-treated NCM460 cells. Flow cytometry and immunoblot assays were performed to confirm the effects of morroniside on the apoptosis of LPS-treated NCM460 cells. Quantitative polymerase chain reaction and enzyme-linked-immunosorbent serologic assays were performed to confirm the effects of morroniside on inflammatory and oxidative stress by measuring the levels of tumor necrosis factor-alpha, interleukin-113, IL-6, superoxide dismutase, malondialdehyde, total antioxi-dant capacity, and myeloperoxidase. In addition, immunoblot and immunofluorescence assays were performed to detect the effects of morroniside on NLRP3 and NF-KB pathways.","Results: Monosine attenuated LPS-induced injury of NCM460 cells. Monosine reduced LPS-induced inflammation in NCM460 cells. In addition, morroniside reduced LPS-induced oxidative stress in NCM460 cells. Mechanically, morroniside suppressed NLRP3 and NF-KB pathways, and alleviated LPS-induced inflammatory and oxidative stress in IBD.","Conclusion: Morroniside could serve as a promising drug for treating IBD. (c) 2022 Codon Publications. Published by Codon Publications."]

inflammatory and oxidative stress Iinflammatory bowel disease (IBD) Morroniside NLRP3

SCI

English

Corresponding

Allergy ; Immunology

Allergy ; Immunology

WOS:000950568400012

CODON PUBLICATIONS

IMMUNOLOGY

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Dept Pathol, Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China
3.Jinan Univ, Shenzhen Peoples Hosp, Dept Tradit Chinese Med, Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China
4.Shenzhen Second Peoples Hosp, Dept Pathol, Shenzhen, Guangdong, Peoples R China
5.Jinan Univ, Shenzhen Peoples Hosp, Dept Radiol, Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China
6.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Pathol, Shen Zhen, Peoples R China
7.Southern Univ Sci & Technol, Affiliated Hosp 1, 1017 Dongmen North Rd, Shen Zhen, Peoples R China

Zhang, Shifen,Lai, Qiaohua,Liu, Liming,et al. Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-KB signaling pathways[J]. ALLERGOLOGIA ET IMMUNOPATHOLOGIA,2022,50(6).

Zhang, Shifen,Lai, Qiaohua,Liu, Liming,Yang, Yajie,&Wang, Juan.(2022).Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-KB signaling pathways.ALLERGOLOGIA ET IMMUNOPATHOLOGIA,50(6).

Zhang, Shifen,et al."Morroniside alleviates lipopolysaccharide-induced inflammatory and oxidative stress in inflammatory bowel disease by inhibiting NLRP3 and NF-KB signaling pathways".ALLERGOLOGIA ET IMMUNOPATHOLOGIA 50.6(2022).