Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells

Chen, Pengchen1,2; Chen, Junhui2; Zhang, Wei2; Tang, Li1; Cheng, Guangqing3; Li, Huiying1; Fan, Tianyun1; Wang, Jigang1,2,3; Zhong, Wenbin1; Song, Yali1

2023-04-15

10.1016/j.ecoenv.2023.114725

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY   Impact Factor & Quartile

0147-6513

1090-2414

Tributyltin chloride (TBTCL) is a widely used fungicide and heat stabilizer in compositions of PVC. TBTCL has been detected in human bodies and potentially causes harmful effects on humans' thyroid, cardiovascular and other organs. As one of the first examples of endocrine disruptors, the toxicity effects of TBTCL on the male reproduction system have aroused concerns. However, the potential cellular mechanisms are not fully explored. In the current study, by using Sertoli cells, a critical regulator of spermatogenesis as a cell model, we showed that with 200 nM exposure for 24 h, TBTCL causes apoptosis and cell cycle arrest. RNA sequencing analyses suggested that TBTCL probably activates endoplasmic reticulum (ER) stress, and disrupts autophagy. Biochemical analysis showed that TBTCL indeed induces ER stress and the dysregulation of autophagy. Interestingly, activation of ER stress and inhibition of autophagy is responsible for TBTCL-induced apoptosis and cell cycle arrest. Our results thus uncovered a novel insight into the cellular mechanisms for TBTCL-induced toxicology in Sertoli cells.

Tributyltin chloride Sertoli cell Endoplasmic reticulum stress Autophagy

SCI

English

Corresponding

China Postdoctoral Science Foundation[K122292027] ; National Key Research and Development Program of China["2020YFA0908000","2022YFC2303600"] ; Innovation Team and Talents Cultivation Program of National Admin- istration of Traditional Chinese Medicine[ZYYCXTD-C-202002] ; National Natural Science Foundation of China["82141001","82274182","82074098","82173914"] ; Science and Technology Foundation of Shenzhen["JCYJ20210324115800001","JCYJ20220818102613029"] ; Shenzhen Governmental Sustainable Development Fund[KCXFZ2020122117 3612034] ; Shenzhen key Laboratory of Kidney Diseases[ZDSY S201504301616234] ; Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties[SZGSP001] ; Guangdong-Dongguan Joint Fund Regional Cultivation Project[2021B1515140033] ; Dongguan Science and Technology of Social Development Program[20221800905732]

Environmental Sciences & Ecology ; Toxicology

Environmental Sciences ; Toxicology

WOS:000955592300001

ACADEMIC PRESS INC ELSEVIER SCIENCE

ENVIRONMENT/ECOLOGY

Web of Science

1.Postdoctoral Innovat Practice Base Southern Med Un, Dongguan Maternal & Child Hlth Care Hosp, Dongguan 523125, Guangdong, Peoples R China
2.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Artemisinin Res Ctr, Beijing 100700, Peoples R China

Chen, Pengchen,Chen, Junhui,Zhang, Wei,et al. Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,255.

Chen, Pengchen.,Chen, Junhui.,Zhang, Wei.,Tang, Li.,Cheng, Guangqing.,...&Song, Yali.(2023).Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,255.

Chen, Pengchen,et al."Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 255(2023).