| Title | Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems |
| Author | |
| Corresponding Author | Guo, Qiongyu; Xi, Lei |
| Publication Years | 2023-03-01
|
| DOI | |
| Source Title | |
| ISSN | 1863-8880
|
| EISSN | 1863-8899
|
| Abstract | Organs maintain their unique functions through a variety of distinctive vessel systems. However, examination of multiset vessels over the entire organ still remains challenging due to the difficulties of assigning multiple vessel systems and labor-intensive imaging reconstruction technologies. Here, an organ-level photoacoustic microscopic imaging platform, named Organ-PAM, with a micron-scale spatial resolution and ultra-large field of view (FOV), which can achieve a lateral and axial FOV of up to approximate to 81 cm(2) and approximate to 30 mm, respectively, is presented. With the assistance of whole-organ decellularization, optical clearing, and a specifically designed photoacoustic gradient concentration differential angiographic pipeline, the visualization of up to four sets of vessel systems inside diversified organs is successfully achieved with multiple scales. In addition, quantitative analyses of vessel systems in both healthy livers and kidneys as well as cancerous livers with exogenous transplanted tumors are conducted at different stages. Thus, the platform enables high-efficiency multiset vessel imaging, recognition, and quantification of different organs, providing critical insights into distinct vessel systems under varying pathological conditions. |
| Keywords | |
| URL | [Source Record] |
| Indexed By | |
| Language | English
|
| SUSTech Authorship | First
; Corresponding
|
| Funding Project | National Natural Science Foundation of China["62022037","62105140","61775028","81571722","61528401","81971764"]
; Department of Science and Technology of Guangdong Province["2019ZT08Y191","SZBL2020090501013","2022B1212010003"]
; Guangdong Provincial Department of Education[2021ZDZX1064]
; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003]
; Shenzhen Science and Technology Program["KQTD20190929172743294","JCYJ20200109141222892","RCBS20210706092213005","JCYJ20220818101403008"]
; Southern University of Science and Technology[PDJH2021C008]
; Innovation Committee of Shenzhen Municipality[JCYJ20200109141638004]
|
| WOS Research Area | Optics
; Physics
|
| WOS Subject | Optics
; Physics, Applied
; Physics, Condensed Matter
|
| WOS Accession No | WOS:000952247000001
|
| Publisher | |
| Data Source | Web of Science
|
| Citation statistics |
Cited Times [WOS]:0
|
| Document Type | Journal Article |
| Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/523955 |
| Department | Department of Biomedical Engineering |
| Affiliation | 1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China 3.Shenzhen Bay Lab, Shenzhen 518132, Guangdong, Peoples R China |
| First Author Affilication | Department of Biomedical Engineering |
| Corresponding Author Affilication | Department of Biomedical Engineering; Southern University of Science and Technology |
| First Author's First Affilication | Department of Biomedical Engineering |
| Recommended Citation GB/T 7714 |
Zhang, Junning,Peng, Dinglu,Qin, Wei,et al. Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems[J]. LASER & PHOTONICS REVIEWS,2023.
|
| APA |
Zhang, Junning.,Peng, Dinglu.,Qin, Wei.,Qi, Weizhi.,Liu, Xiaoya.,...&Xi, Lei.(2023).Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems.LASER & PHOTONICS REVIEWS.
|
| MLA |
Zhang, Junning,et al."Organ-PAM: Photoacoustic Microscopy of Whole-organ Multiset Vessel Systems".LASER & PHOTONICS REVIEWS (2023).
|
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