New Set of Isobaric Labeling Reagents for Quantitative 16Plex Proteomics

Ning, Xiaolian1,2; Li, Qidan2; Zi, Jin2,3; Mei, Zhanlong2; Liu, Jie2; Zhang, Yuxing2; Bi, Mao4; Ren, Yan3,5; Liu, Xingang6; Lv, Chao6; Yao, Hequan7; Sun, Jianguo7; Rao, Feng; Li, Shuwei6,7; Liu, Siqi2,3

2023-03-01

10.1021/acs.analchem.3c00235

ANALYTICAL CHEMISTRY   Impact Factor & Quartile

0003-2700

1520-6882

Peptide labeling by isobaric tags is a powerful approach for the relative quantitative analysis of proteomes in multiple groups. There has been a revolution in the innovation of new isobaric reagents; however, great effort is being made to expand simultaneous labeling groups to identify more labeled peptides and reduce reporter ion signal suppression. We redesigned the original chemical structure of the deuterium isobaric amine-reactive tag developed in our laboratory. We optimized the synthetic pathway to create a new set of 16-plex isobaric tags (IBT-16plex). The novel reagent enabled almost complete labeling of peptides within 90 min, with all labeling reporter ions exhibiting comparable MS/MS signals. Compared to a typical 16plex reagent, TMTpro16plex, the peptides and proteins identified by IBT-16plex in trypsinized HeLa cells were significantly increased by 14.8 and 8.6%, respectively. Moreover, differences in peptide abundance within 10-fold among multiple groups were barely suppressed in IBT16plex, whereas the dynamic range in TMTpro-16plex-labeled groups was smaller. After quantitative examination of MCF7 cell proteins, IBT-16plex was confirmed as feasible and useful for evaluating protein responses of glucose-starved MCF7 cells to a glucose-rich medium.

SCI

English

Others

National Key R&D Program of China[2022YFA1304500] ; null[2020YFE0202200]

Chemistry

Chemistry, Analytical

WOS:000959434300001

AMER CHEMICAL SOC

CHEMISTRY

Web of Science

1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
2.BGI Shenzhen, Shenzhen 518083, Guangdong, Peoples R China
3.Chinese Acad Sci, Zhejiang Canc Hosp, Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China
4.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
5.Shanghai Univ Tradit Chinese Med, Expt Ctr Sci & Technol, Shanghai 201203, Peoples R China
6.Nanjing Apoll Biotech Inc, Nanjing 210033, Jiangsu, Peoples R China
7.China Pharmaceut Univ, Nanjing 210009, Jiangsu, Peoples R China

Ning, Xiaolian,Li, Qidan,Zi, Jin,et al. New Set of Isobaric Labeling Reagents for Quantitative 16Plex Proteomics[J]. ANALYTICAL CHEMISTRY,2023.

Ning, Xiaolian.,Li, Qidan.,Zi, Jin.,Mei, Zhanlong.,Liu, Jie.,...&Liu, Siqi.(2023).New Set of Isobaric Labeling Reagents for Quantitative 16Plex Proteomics.ANALYTICAL CHEMISTRY.

Ning, Xiaolian,et al."New Set of Isobaric Labeling Reagents for Quantitative 16Plex Proteomics".ANALYTICAL CHEMISTRY (2023).