A cytoderm metabolic labeling AIEgen for rapid detection and intracellular ablation of Mycobacterium tuberculosis

Dai, Guiqin1,3; Luo, Yuan2; Liao, Mingfeng1; Zhang, Pengfei2; Pan, Hong2; Yin, Ting2,3; Yang, Qianting1; Zheng, Sirui2,3; Liao, Jianhong2; Liu, Deliang1; He, Zhuojun1,3; Zhao, Wenchang3; Song, Lijun3; Zhao, Pengfei1,4; Cai, Lintao2; Zhang, Zheng1; Zheng, Mingbin1,2,3

2023-02-15

10.1016/j.xcrp.2022.101238

CELL REPORTS PHYSICAL SCIENCE   Impact Factor & Quartile

2666-3864

Latent Mycobacterium tuberculosis (Mtb) shielded in macrophages is hard to detect and eradicate, causing lethal respiratory failure and sys-temic inflammation. Here, we synthesize an aggregation-induced emission luminogen (AIEgen) with metabolic labeling and in situ photodynamic scavenging functions via click chemistry. By partici-pating in cytoderm peptidoglycan biosynthesis, the wash-free AIEgen presents quick (10 min) and selective metabolic labeling to live Mtb and obtains 50-fold better detection sensitivity of tuberculosis compared with acid-fast bacilli (AFBs). Notably, the AIEgen demon-strates accurate (>96%), sensitive (>96%), and specific (100%) clinical tuberculosis diagnosis using bronchoalveolar lavage fluid (BALF) and sputum samples. Upon low-power light irradiation, the AIEgen gener-ates lethal-dose reactive oxygen species (ROS) to break the bacterial cytoderm and significantly improves the sensitivity of Mtb to rifam-picin via photodynamic ablation. The AIEgen is a clinically oriented diagnostic for tuberculosis mass screening in regions with low income and a high tuberculosis burden and shows excellent potential for photodynamic ablation of drug-resistant tuberculosis.

SCI

English

First ; Corresponding

National Natural Science Foundation of China["81971749","81901864","62005176","82172286"] ; National Key Research and Devel- opment Program of China[2019YFE0198700] ; Guangdong Provincial Key Area RD Program[2020B1111540001] ; Shenzhen Science and Technology Program["JCYJ20200109114616534","JCYJ20210324115611032","JCYJ20210324115607020","JCYJ20210324131603008"] ; Zhuhai Innovation and Entrepreneurship Team Proj- ect[ZH01110405180056PWC] ; China Postdoctoral Science Foundation[2021M693313]

Chemistry ; Energy & Fuels ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Energy & Fuels ; Materials Science, Multidisciplinary ; Physics, Multidisciplinary

WOS:000961296200001

ELSEVIER

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Inst Hepatol,Natl Clin Res Ctr Infect Dis,Rheumato, Shenzhen 518112, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Adv Technol, Guangdong Key Lab Nanomed, CAS HK Joint Lab Biomat, Shenzhen 518055, Peoples R China
3.Guangdong Med Univ, Guangdong Key Lab Res & Dev Nat Drugs, Dongguan 523808, Peoples R China
4.Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen Key Lab Photon & Biophoton, Key Lab Optoelect Devices & Syst,Minist Educ & Gua, Shenzhen 518060, Peoples R China

Dai, Guiqin,Luo, Yuan,Liao, Mingfeng,et al. A cytoderm metabolic labeling AIEgen for rapid detection and intracellular ablation of Mycobacterium tuberculosis[J]. CELL REPORTS PHYSICAL SCIENCE,2023,4(2).

Dai, Guiqin.,Luo, Yuan.,Liao, Mingfeng.,Zhang, Pengfei.,Pan, Hong.,...&Zheng, Mingbin.(2023).A cytoderm metabolic labeling AIEgen for rapid detection and intracellular ablation of Mycobacterium tuberculosis.CELL REPORTS PHYSICAL SCIENCE,4(2).

Dai, Guiqin,et al."A cytoderm metabolic labeling AIEgen for rapid detection and intracellular ablation of Mycobacterium tuberculosis".CELL REPORTS PHYSICAL SCIENCE 4.2(2023).