Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery-associated acute kidney injury

Bai, Yunpeng1,2; Huang, Wendong1; Jiang, Xinyi3,4,5; Xu, Wang3,4,6; Li, Ying3,4; Wang, Yirong3,4,7; Huang, Sumei1,8; Wu, Kunyong1,8; Hu, Linhui2; Chen, Chunbo9,10,11

2023-06-15

10.1002/rcm.9504

RAPID COMMUNICATIONS IN MASS SPECTROMETRY   Impact Factor & Quartile

0951-4198

1097-0231

RationaleCardiac surgery-associated acute kidney injury (CSA-AKI) is a prevalent complication of cardiac surgery, which may be associated with a great risk of developing chronic kidney disease and mortality. This study aimed to investigate the possible links between gut microbiota metabolism and CSA-AKI. MethodsA prospective cohort of patients who underwent cardiac surgery was continuously recruited, who were further divided into CSA-AKI group and Non-AKI group based on clinical outcomes. Their faecal and plasma samples were collected before surgery and were separately analysed by nontargeted and targeted metabolomics. The differential metabolites related to CSA-AKI were screened out using statistical methods, and altered metabolic pathways were determined by examining the Kyoto Encyclopedia of Genes and Genomes database. ResultsNearly 1000 faecal metabolites were detected through high-resolution mass spectrometry (MS) and bioinformatics at high and mid confidence levels, and 49 differential metabolites at high confidence level may perform essential biological functions and provide potential diagnostic indicators. Compared with the Non-AKI group, the patients in the CSA-AKI group displayed dramatic changes in gut microbiota metabolism, including amino acid metabolism, nicotinate and nicotinamide metabolism, purine metabolism and ATP-binding cassette (ABC) transporters. Meanwhile, 188 plasma metabolites were identified and quantified by tandem MS, and 34 differential plasma metabolites were screened out between the two groups using univariate statistical analysis. These differential plasma metabolites were primarily enriched in the following metabolic pathways: sulphur metabolism, amino acid biosynthesis, tryptophan metabolism and ABC transporters. Furthermore, the content of indole metabolites in the faecal and plasma samples of the CSA-AKI group was higher than that of the Non-AKI group. ConclusionsPatients with CSA-AKI may have dysbiosis of their intestinal microbiota and metabolic abnormalities in their gut system before cardiac surgery. Thus, some metabolites and related metabolic pathways may be potential biomarkers and new therapeutic targets for the disease.

SCI

English

Corresponding

National Natural Science Foundation of China[82172162] ; Major Program of Summit Project, Guangdong Province High-level Hospital Construction Project of Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences[DFJH2020028] ; Outstanding Young Medical Talents in Guangdong Province[KJ012019445] ; Science and Technology Planning Project of Guangdong Province of China["mmkj2020025","2021S0024"] ; Guangdong Basic and Applied Basic Research Foundation[2022A1515220065] ; Natural Science Foundation of Guangdong Province of China[2021A1515010884] ; Medical Scientific Research Foundation of Guangdong Province of China["A2017490","B2018061"] ; Maoming Science and Technology Program[2019018023] ; High-level Hospital Construction Research Project of Maoming People's Hospital[zx2020016]

Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy

Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy

WOS:000963795300001

WILEY

CHEMISTRY

Web of Science

1.Maoming Peoples Hosp, Ctr Sci Res, Maoming, Peoples R China
2.Maoming Peoples Hosp, Dept Crit Care Med, Maoming, Peoples R China
3.Southern Med Univ, Guangdong Prov Peoples Hosp, Guangdong Acad Med Sci, Dept Intens Care Unit Cardiovasc Surg, Guangzhou, Peoples R China
4.Guangdong Prov Peoples Hosp, Guangdong Acad Med Sci, Guangdong Cardiovasc Inst, Guangzhou, Peoples R China
5.South China Univ Technol, Sch Med, Guangzhou, Peoples R China
6.Southern Med Univ, Sch Clin Med 2, Guangzhou, Peoples R China
7.South China Univ Technol, Sch Biol & Biol Engn, Guangzhou, Peoples R China
8.Maoming Peoples Hosp, Biol Resource Ctr, Maoming, Peoples R China
9.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Crit Care M, Shenzhen, Peoples R China
10.Maoming Peoples Hosp, Dept Emergency Med, Maoming, Peoples R China
11.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Crit Care M, Shenzhen 518000, Peoples R China

Bai, Yunpeng,Huang, Wendong,Jiang, Xinyi,et al. Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery-associated acute kidney injury[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2023,37(11).

Bai, Yunpeng.,Huang, Wendong.,Jiang, Xinyi.,Xu, Wang.,Li, Ying.,...&Chen, Chunbo.(2023).Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery-associated acute kidney injury.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,37(11).

Bai, Yunpeng,et al."Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery-associated acute kidney injury".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 37.11(2023).