中文版 | English
Title

Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force

Author
Corresponding AuthorBi, Long; Pei, Guoxian
Publication Years
2023-03-01
DOI
Source Title
ISSN
1944-8244
EISSN
1944-8252
Volume15Issue:13
Abstract
Groove patterns are widely used in material surface modifications. However, the independent role of ditches/ridges in regulating fibrosis of soft tissues is not well-understood, especially the lack of linkage evidence in vitro and in vivo. Herein, two kinds of combinational microgroove chips with the gradient ditch/ridge width were fabricated by photolithography technology, termed R and G groups, respectively. In group R, the ridge width was 1, 5, 10, and 30 mu m, with a ditch width of 30 mu m; in group G, the groove width was 5, 10, 20, and 30 mu m, and the ridge width was 5 mu m. The effect of microgrooves on the morphology, proliferation, and expression of fibrous markers of stem cells was systematically investigated in vitro. Moreover, thicknesses of fibrous capsules were evaluated after chips were implanted into the muscular pouches of rats for 5 months. The results show that microgrooves have almost no effect on cell proliferation but significantly modulate the morphology of cells and focal adhesions (FAs) in vitro, as well as fibrosis differentiation. In particular, the differentiation of stem cells is attenuated after the intracellular force caused by stress fibers and FAs is interfered by drugs, such as rotenone and blebbistatin. Histological analysis shows that patterns of high intracellular force can apparently stimulate soft tissue fibrosis in vivo. This study not only reveals the specific rules and mechanisms of ditch/ridge regulating stem cell behaviors but also offers insight into tailoring implant surface patterns to induce controlled soft tissue fibrosis.
Keywords
URL[Source Record]
Indexed By
Language
English
SUSTech Authorship
Corresponding
Funding Project
National Key R&D Program of China[2016YFC1100304] ; Shandong Provincial Natural Science Foundation[ZR2022MH071] ; Shenzhen Hong Kong[SGDX20211123114204007] ; National Science Foundation of China[81902202] ; Linyi Municipal Science and Technology Develop-ment Plan[201818015]
WOS Research Area
Science & Technology - Other Topics ; Materials Science
WOS Subject
Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS Accession No
WOS:000959547500001
Publisher
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/524067
DepartmentSchool of Medicine
南方科技大学医院
Affiliation
1.Ait Force Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China
2.Linyi Peoples Hosp, Dept Orthoped Surg, Linyi 276000, Peoples R China
3.Air Force Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China
4.Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200438, Peoples R China
5.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
6.Southern Univ Sci & Technol Hosp, Shenzhen 518055, Peoples R China
Corresponding Author AffilicationSchool of Medicine;  Southern University of Science and Technology Hospital
Recommended Citation
GB/T 7714
Lei, Xing,Miao, Sheng,Wang, Xiuli,et al. Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force[J]. ACS APPLIED MATERIALS & INTERFACES,2023,15(13).
APA
Lei, Xing.,Miao, Sheng.,Wang, Xiuli.,Gao, Yi.,Wu, Hao.,...&Pei, Guoxian.(2023).Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force.ACS APPLIED MATERIALS & INTERFACES,15(13).
MLA
Lei, Xing,et al."Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force".ACS APPLIED MATERIALS & INTERFACES 15.13(2023).
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