Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force

Lei, Xing1,2; Miao, Sheng1,3; Wang, Xiuli; Gao, Yi4,5,6; Wu, Hao1,3; Cheng, Pengzhen1; Song, Yue1; Bi, Long1; Pei, Guoxian1,3,4,5,6

2023-03-01

10.1021/acsami.2c20903

ACS APPLIED MATERIALS & INTERFACES   Impact Factor & Quartile

1944-8244

1944-8252

Groove patterns are widely used in material surface modifications. However, the independent role of ditches/ridges in regulating fibrosis of soft tissues is not well-understood, especially the lack of linkage evidence in vitro and in vivo. Herein, two kinds of combinational microgroove chips with the gradient ditch/ridge width were fabricated by photolithography technology, termed R and G groups, respectively. In group R, the ridge width was 1, 5, 10, and 30 mu m, with a ditch width of 30 mu m; in group G, the groove width was 5, 10, 20, and 30 mu m, and the ridge width was 5 mu m. The effect of microgrooves on the morphology, proliferation, and expression of fibrous markers of stem cells was systematically investigated in vitro. Moreover, thicknesses of fibrous capsules were evaluated after chips were implanted into the muscular pouches of rats for 5 months. The results show that microgrooves have almost no effect on cell proliferation but significantly modulate the morphology of cells and focal adhesions (FAs) in vitro, as well as fibrosis differentiation. In particular, the differentiation of stem cells is attenuated after the intracellular force caused by stress fibers and FAs is interfered by drugs, such as rotenone and blebbistatin. Histological analysis shows that patterns of high intracellular force can apparently stimulate soft tissue fibrosis in vivo. This study not only reveals the specific rules and mechanisms of ditch/ridge regulating stem cell behaviors but also offers insight into tailoring implant surface patterns to induce controlled soft tissue fibrosis.

groove patterns stem cells intracellular force focal adhesions fibrosis

SCI

English

Corresponding

National Key R&D Program of China[2016YFC1100304] ; Shandong Provincial Natural Science Foundation[ZR2022MH071] ; Shenzhen Hong Kong[SGDX20211123114204007] ; National Science Foundation of China[81902202] ; Linyi Municipal Science and Technology Develop-ment Plan[201818015]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000959547500001

AMER CHEMICAL SOC

Web of Science

1.Ait Force Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China
2.Linyi Peoples Hosp, Dept Orthoped Surg, Linyi 276000, Peoples R China
3.Air Force Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China
4.Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200438, Peoples R China
5.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
6.Southern Univ Sci & Technol Hosp, Shenzhen 518055, Peoples R China

Lei, Xing,Miao, Sheng,Wang, Xiuli,et al. Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force[J]. ACS APPLIED MATERIALS & INTERFACES,2023,15(13).

Lei, Xing.,Miao, Sheng.,Wang, Xiuli.,Gao, Yi.,Wu, Hao.,...&Pei, Guoxian.(2023).Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force.ACS APPLIED MATERIALS & INTERFACES,15(13).

Lei, Xing,et al."Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force".ACS APPLIED MATERIALS & INTERFACES 15.13(2023).