Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation

Sun，Avery Rui1,4,5; Sun，Qili1; Wang，Yansong1; Hu，Liqiu1; Wu，Yutong1; Ma，Fenbo1; Liu，Jiayi1; Pang，Xiangchao1,6; Tang，Bin1,2,3

2023-12-01

10.1186/s40824-023-00361-2

Biomaterials Research   Impact Factor & Quartile

2055-7124

Background: Titanium (Ti) is now widely used as implant material due to its excellent mechanical properties and superior biocompatibilities, while its inert bioactivities might lead to insufficient osseointegration, and limit its performance in dental applications. Methods: We introduced a robust and simple approach of modifying titanium surfaces with polysaccharide complexes. Titanium samples were subjected to hydrothermal treatment to create a uniform porous structure on the surface, followed by coating with a bioinspired and self-assembly polydopamine layer. Strontium Eucommia Ulmoides Polysaccharide (EUP-Sr) complexes are then introduced to the polydopamine-coated porous titanium. Multiple morphological and physiochemical characterizations are employed for material evaluation, while cell proliferation and gene expression tests using macrophages, primary alveolar bone osteoblasts, and vascular endothelial cells are used to provide an overall insight into the functions of the product. The significances of statistical differences were analyzed using student’s t-test. Results: Microscopic and spectrometric characterizations confirmed that the Ti surface formed a porous structure with an adequate amount of EUP-Sr loading. The attachment was attributed to hydrogen bonding between the ubiquitous glycosidic linkage of the polysaccharide complex and the ring structure of polydopamine, yet the loaded EUP-Sr complex can be gradually released, consequently benefiting the neighboring microenvironment. Cell experiments showed no cytotoxicity of the material, and the product showed promising anti-inflammation, osseointegration, and angiogenesis properties, which were further confirmed by in vivo evaluations. Conclusion: We believe the EUP-Sr modified titanium implant is a promising candidate to be used in dental applications with notable osteoimmunomodulation and angiogenesis functions. And the novel technique proposed in this study would benefit the modification of metal/inorganic surfaces with polysaccharides for future research.

Anti-inflammation Biological interfaces Osseointegration Osteoimmunomodulation Polydopamine Strontium Eucommia Ulmoides polysaccharides Titanium dental implant

SCI

English

First ; Corresponding

National Natural Science Foundation of China-China Academy of General Technology Joint Fund for Basic Research[11872200];Medical Science and Technology Foundation of Guangdong Province[2017B030301018];

WOS:000949397700001

2-s2.0-85150725164

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Guangdong,China
3.Shenzhen Key Laboratory of Cell Microenvironment,Shenzhen,China
4.Department of Biomedical Engineering,College of Design and Engineering,National University of Singapore,Singapore,117583,Singapore
5.Mechanobiology Institute (MBI),National University of Singapore,Singapore,117411,Singapore
6.College of Materials Science and Engineering,Central South University of Forestry and Technology,Changsha,410004,China

Sun，Avery Rui,Sun，Qili,Wang，Yansong,et al. Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation[J]. Biomaterials Research,2023,27(1).

Sun，Avery Rui.,Sun，Qili.,Wang，Yansong.,Hu，Liqiu.,Wu，Yutong.,...&Tang，Bin.(2023).Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation.Biomaterials Research,27(1).

Sun，Avery Rui,et al."Surface modifications of titanium dental implants with strontium eucommia ulmoides to enhance osseointegration and suppress inflammation".Biomaterials Research 27.1(2023).