High-efficiency degradation of methomyl by the novel bacterial consortium MF0904: Performance, structural analysis, metabolic pathways, and environmental bioremediation

Pang，Shimei1,2,3; Lin，Ziqiu4; Chen，Wen Juan1,2; Chen，Shao Fang1,2; Huang，Yaohua1,2; Lei，Qiqi1,2; Bhatt，Pankaj5; Mishra，Sandhya6; Chen，Shaohua1,2; Wang，Huishan1,2

2023-06-15

10.1016/j.jhazmat.2023.131287

Journal of Hazardous Materials   Impact Factor & Quartile

0304-3894

1873-3336

Methomyl is a widely used carbamate pesticide, which has adverse biological effects and poses a serious threat to ecological environments and human health. Several bacterial isolates have been investigated for removing methomyl from environment. However, low degradation efficiency and poor environmental adaptability of pure cultures severely limits their potential for bioremediation of methomyl-contaminated environment. Here, a novel microbial consortium, MF0904, can degrade 100% of 25 mg/L methomyl within 96 h, an efficiency higher than that of any other consortia or pure microbes reported so far. The sequencing analysis revealed that Pandoraea, Stenotrophomonas and Paracoccus were the predominant members of MF0904 in the degradation process, suggesting that these genera might play pivotal roles in methomyl biodegradation. Moreover, five new metabolites including ethanamine, 1,2-dimethyldisulfane, 2-hydroxyacetonitrile, N-hydroxyacetamide, and acetaldehyde were identified using gas chromatography–mass spectrometry, indicating that methomyl could be degraded firstly by hydrolysis of its ester bond, followed by cleavage of the C–S ring and subsequent metabolism. Furthermore, MF0904 can successfully colonize and substantially enhance methomyl degradation in different soils, with complete degradation of 25 mg/L methomyl within 96 and 72 h in sterile and nonsterile soil, respectively. Together, the discovery of microbial consortium MF0904 fills a gap in the synergistic metabolism of methomyl at the community level and provides a potential candidate for bioremediation applications.

Biodegradation Consortium Metabolic pathways Methomyl Soil remediation

English

Others

Science and Technology Planning Project of Guangdong Province[20210205];Science and Technology Planning Project of Guangdong Province[2021B1212040008];Natural Science Foundation of Guangdong Province[2022A1515010422];Natural Science Foundation of Guangdong Province[2023A1515012552];National Natural Science Foundation of China[32200025];

ENGINEERING

2-s2.0-85151433914

Scopus

1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Integrative Microbiology Research Centre,South China Agricultural University,Guangzhou,510642,China
2.Guangdong Laboratory for Lingnan Modern Agriculture,College of Plant Protection,South China Agricultural University,Guangzhou,510642,China
3.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China
4.The Hong Kong University of Science and Technology,Hong Kong,Hong Kong
5.Department of Agricultural & Biological Engineering,Purdue University,West Lafayette,47906,United States
6.Environmental Technologies Division,CSIR-National Botanical Research Institute,Lucknow,Rana Pratap Marg,226001,India

Pang，Shimei,Lin，Ziqiu,Chen，Wen Juan,et al. High-efficiency degradation of methomyl by the novel bacterial consortium MF0904: Performance, structural analysis, metabolic pathways, and environmental bioremediation[J]. Journal of Hazardous Materials,2023,452.

Pang，Shimei.,Lin，Ziqiu.,Chen，Wen Juan.,Chen，Shao Fang.,Huang，Yaohua.,...&Wang，Huishan.(2023).High-efficiency degradation of methomyl by the novel bacterial consortium MF0904: Performance, structural analysis, metabolic pathways, and environmental bioremediation.Journal of Hazardous Materials,452.

Pang，Shimei,et al."High-efficiency degradation of methomyl by the novel bacterial consortium MF0904: Performance, structural analysis, metabolic pathways, and environmental bioremediation".Journal of Hazardous Materials 452(2023).