Highly efficient and non-doped red conjugated polymer dot for photostable cell imaging

Wu，Mengqi1; Wei，Qidong1; Xian，Caihong1; Dai，Chunlei1; He，Xuehan2; Wu，Changfeng3; Sun，Guoming4; Chen，Lei1

2023-06-01

10.1016/j.cclet.2022.107867

Chinese Chemical Letters   Impact Factor & Quartile

1001-8417

By introducing a naphthothiadiazole (NT) unit as the main building block, a non-doped and red emissive conjugated polymer poly(9,9-dihexylfluorene-alt-naphthothiadiazole) (PFNT) is readily obtained through a two-step synthesis. Since the NT unit has a large twist angle with its neighboring segment, the aggregation-induced quenching (AIQ) effect of PFNT can be effectively suppressed in the condensed state. As a result, the corresponding PFNT polymer dot (Pdot) exhibits a high fluorescence quantum yield of 53.2% with peak emission at 616 nm, which is one of the most efficient red Pdots known. PFNT Pdot shows good biocompatibility and can be employed for living cell fluorescent imaging with high brightness. It also can be used for specific subcellular organelle imaging through immunofluorescence labeling. Furthermore, the PFNT Pdot demonstrates much better photostability for long-time cell fluorescence imaging than commercial red dyes. The high performances of PFNT Pdot make it a promising fluorescent probe for practical bioapplications.

Cell imaging Conjugated polymer dot Naphthothiadiazole Non-doped Photostability

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CHEMISTRY

2-s2.0-85144712089

Scopus

1.School of Biomedical Engineering,Sun Yat-sen University,Shenzhen,518107,China
2.Teaching and Experiment Centre,Sun Yat-sen University,Shenzhen,518107,China
3.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
4.College of Chemistry and Environmental Science,Hebei University,Affiliated Hospital of Hebei University,Baoding,071002,China

Wu，Mengqi,Wei，Qidong,Xian，Caihong,et al. Highly efficient and non-doped red conjugated polymer dot for photostable cell imaging[J]. Chinese Chemical Letters,2023,34(6).

Wu，Mengqi.,Wei，Qidong.,Xian，Caihong.,Dai，Chunlei.,He，Xuehan.,...&Chen，Lei.(2023).Highly efficient and non-doped red conjugated polymer dot for photostable cell imaging.Chinese Chemical Letters,34(6).

Wu，Mengqi,et al."Highly efficient and non-doped red conjugated polymer dot for photostable cell imaging".Chinese Chemical Letters 34.6(2023).