中文版 | English
Title

Structure and mechanism of the plant RNA polymerase V

Author
Corresponding AuthorDu,Jiamu
Joint first authorXie,Guohui; Du,Xuan; Hu,Hongmiao
Publication Years
2023-03-24
DOI
Source Title
ISSN
0036-8075
EISSN
1095-9203
Volume379Issue:6638Pages:1209-1213
Abstract

In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a double-stranded DNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The nontemplate DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3′-5′ cleavage, which likely underpins Pol V’s high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking, which may be important for Pol V’s retention on chromatin to serve its function in tethering downstream factors for RNA-directed DNA methylation.

URL[Source Record]
Indexed By
Language
English
Important Publications
NI Journal Papers
SUSTech Authorship
First ; 共同第一 ; Corresponding
ESI Research Field
BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;COMPUTER SCIENCE;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;ENGINEERING
Citation statistics
Cited Times [WOS]:2
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/524192
DepartmentSUSTech-Peking University National Institute of Plants and Food
生命科学学院
生命科学学院_生物系
Affiliation
1.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Institute of Plant and Food Science,Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
2.Department of Biochemistry and Molecular Biology,International Cancer Center,Shenzhen University Medical School,Shenzhen,518060,China
3.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101,China
4.Department of Molecular,Cell and Developmental Biology,University of California at Los Angeles,Los Angeles,90095,United States
5.Howard Hughes Medical Institute,University of California at Los Angeles,Los Angeles,90095,United States
First Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Corresponding Author AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
First Author's First AffilicationSUSTech-Peking University National Institute of Plants and Food;  Department of Biology;  School of Life Sciences
Recommended Citation
GB/T 7714
Xie,Guohui,Du,Xuan,Hu,Hongmiao,et al. Structure and mechanism of the plant RNA polymerase V[J]. Science,2023,379(6638):1209-1213.
APA
Xie,Guohui.,Du,Xuan.,Hu,Hongmiao.,Li,Sisi.,Cao,Xiaofeng.,...&Du,Jiamu.(2023).Structure and mechanism of the plant RNA polymerase V.Science,379(6638),1209-1213.
MLA
Xie,Guohui,et al."Structure and mechanism of the plant RNA polymerase V".Science 379.6638(2023):1209-1213.
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