Structure and mechanism of the plant RNA polymerase V
|Joint first author||Xie，Guohui; Du，Xuan; Hu，Hongmiao|
In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a double-stranded DNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The nontemplate DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3′-5′ cleavage, which likely underpins Pol V’s high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking, which may be important for Pol V’s retention on chromatin to serve its function in tethering downstream factors for RNA-directed DNA methylation.
NI Journal Papers
First ; 共同第一 ; Corresponding
|ESI Research Field|
BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;COMPUTER SCIENCE;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;ENGINEERING
Cited Times [WOS]:2
|Document Type||Journal Article|
|Department||SUSTech-Peking University National Institute of Plants and Food|
1.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Institute of Plant and Food Science,Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
2.Department of Biochemistry and Molecular Biology,International Cancer Center,Shenzhen University Medical School,Shenzhen,518060,China
3.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101,China
4.Department of Molecular,Cell and Developmental Biology,University of California at Los Angeles,Los Angeles,90095,United States
5.Howard Hughes Medical Institute,University of California at Los Angeles,Los Angeles,90095,United States
|First Author Affilication||SUSTech-Peking University National Institute of Plants and Food; Department of Biology; School of Life Sciences|
|Corresponding Author Affilication||SUSTech-Peking University National Institute of Plants and Food; Department of Biology; School of Life Sciences|
|First Author's First Affilication||SUSTech-Peking University National Institute of Plants and Food; Department of Biology; School of Life Sciences|
Xie，Guohui,Du，Xuan,Hu，Hongmiao,et al. Structure and mechanism of the plant RNA polymerase V[J]. Science,2023,379(6638):1209-1213.
Xie，Guohui.,Du，Xuan.,Hu，Hongmiao.,Li，Sisi.,Cao，Xiaofeng.,...&Du，Jiamu.(2023).Structure and mechanism of the plant RNA polymerase V.Science,379(6638),1209-1213.
Xie，Guohui,et al."Structure and mechanism of the plant RNA polymerase V".Science 379.6638(2023):1209-1213.
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