Influence of Extremely High Pressure and Oxygen on Hydrocarbon-Enriched Microbial Communities in Sediments from the Challenger Deep, Mariana Trench

Liu，Ying1; Chen，Songze2; Xie，Zhe1; Zhang，Li1; Wang，Jiahua1; Fang，Jiasong1,3,4

2023-03-01

10.3390/microorganisms11030630

Microorganisms   Impact Factor & Quartile

2076-2607

Recent studies reported that highly abundant alkane content exists in the ~11,000 m sediment of the Mariana Trench, and a few key alkane-degrading bacteria were identified in the Mariana Trench. At present, most of the studies on microbes for degrading hydrocarbons were performed mainly at atmospheric pressure (0.1 MPa) and room temperature; little is known about which microbes could be enriched with the addition of n-alkanes under in-situ environmental pressure and temperature conditions in the hadal zone. In this study, we conducted microbial enrichments of sediment from the Mariana Trench with short-chain (SCAs, C–C) or long-chain (LCAs, C–C) n-alkanes and incubated them at 0.1 MPa/100 MPa and 4 °C under aerobic or anaerobic conditions for 150 days. Microbial diversity analysis showed that a higher microbial diversity was observed at 100 MPa than at 0.1 MPa, irrespective of whether SCAs or LCAs were added. Non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis revealed that different microbial clusters were formed according to hydrostatic pressure and oxygen. Significantly different microbial communities were formed according to pressure or oxygen (p < 0.05). For example, Gammaproteobacteria (Thalassolituus) were the most abundant anaerobic n-alkanes-enriched microbes at 0.1 MPa, whereas the microbial communities shifted to dominance by Gammaproteobacteria (Idiomarina, Halomonas, and Methylophaga) and Bacteroidetes (Arenibacter) at 100 MPa. Compared to the anaerobic treatments, Actinobacteria (Microbacterium) and Alphaproteobacteria (Sulfitobacter and Phenylobacterium) were the most abundant groups with the addition of hydrocarbon under aerobic conditions at 100 MPa. Our results revealed that unique n-alkane-enriched microorganisms were present in the deepest sediment of the Mariana Trench, which may imply that extremely high hydrostatic pressure (100 MPa) and oxygen dramatically affected the processes of microbial-mediated alkane utilization.

anaerobic and aerobic incubation hydrostatic pressure long-period culture Mariana Trench sediments n-alkane-enriched microorganisms short-chain and long-chain n-alkanes

SCI

English

Others

National Natural Science Foundation of China[91951210];National Natural Science Foundation of China[92241303];National Natural Science Foundation of China[92251303];

Microbiology

Microbiology

WOS:000960029000001

MDPI

2-s2.0-85151444437

Scopus

1.Shanghai Engineering Research Center of Hadal Science and Technology,Shanghai Ocean University,Shanghai,200120,China
2.Shenzhen Key Laboratory of Marine Archaea Geo-Omics,Department of Ocean Science and Engineering,Southern University of Science and Technology,Shenzhen,518000,China
3.Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,266000,China
4.Department of Natural Sciences,Hawaii Pacific University,Honolulu,96813,United States

Liu，Ying,Chen，Songze,Xie，Zhe,et al. Influence of Extremely High Pressure and Oxygen on Hydrocarbon-Enriched Microbial Communities in Sediments from the Challenger Deep, Mariana Trench[J]. Microorganisms,2023,11(3).

Liu，Ying,Chen，Songze,Xie，Zhe,Zhang，Li,Wang，Jiahua,&Fang，Jiasong.(2023).Influence of Extremely High Pressure and Oxygen on Hydrocarbon-Enriched Microbial Communities in Sediments from the Challenger Deep, Mariana Trench.Microorganisms,11(3).

Liu，Ying,et al."Influence of Extremely High Pressure and Oxygen on Hydrocarbon-Enriched Microbial Communities in Sediments from the Challenger Deep, Mariana Trench".Microorganisms 11.3(2023).