Gold Nanoparticles Reduce Food Sensation in Caenorhabditis elegans via the Voltage-Gated Channel EGL-19

Wang，Meimei1; Zhang，Zhenzhen2; Sun，Ning3; Yang，Baolin4; Mo，Jihao5; Wang，Daping2; Su，Mingqin1; Hu，Jian1; Wang，Miaomiao6; Wang，Lei7,8

2023

10.2147/IJN.S394666

International journal of nanomedicine   Impact Factor & Quartile

1178-2013

1178-2013

Introduction: The increasing use of gold nanoparticles (Au NPs) in the medical field has raised concerns about the potential adverse effect of Au NPs exposure. However, it is difficult to assess the health risks of Au NPs exposure at the individual organ level using current measurement techniques. Methods: The physical and chemical properties of Au NPs were characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR), and zeta sizer. The RNA-seq data of Au NPs-exposed worms were analyzed. The food intake was measured by liquid culture and Pharyngeal pumping rate. The function of the smell and taste neurons was evaluated by the chemotaxis and avoidance assay. The activation of ASE neurons was analyzed by calcium imaging. The gene expression of ins-22 and egl-19 was obtained from the C. elegans single cell RNA-seq databases. Results: Our data analysis indicated that 62.8% of the significantly altered genes were functional in the nervous system. Notably, developmental stage analysis demonstrated that exposure to Au NPs interfered with animal development by regulating foraging behavior. Also, our chemotaxis results showed that exposure to Au NPs reduced the sensation of C. elegans to NaCl, which was consistent with the decrease in calcium transit of ASEL. Further studies confirmed that the reduced calcium transit was dependent on voltage-gated calcium channel EGL-19. The neuropeptide INS-22 was partially involved in Au NPs-induced NaCl sensation defect. Therefore, we proposed that Au NPs reduced the calcium transit in the ASEL neuron through egl-19-dependent calcium channels. It was partially regulated by the DAF-16 targeting neuropeptide INS-22. Discussion: Our results demonstrate that Au NPs affect food sensation by reducing the calcium transit in ASEL neurons, which further leads to reduced pharynx pumping and feeding defects. The toxicology studies of Au NPs from worms have great potential to guide the usage of Au NPs in the medical field such as targeted drug delivery.

ASEL neuron Caenorhabditis elegans developmental toxicity gold nanoparticles RNA sequencing voltage-gated calcium channel

SCI

English

Others

National Natural Science Foundation of China[22176002] ; Young Scientists Fund of the National Natural Science Foundation of China[82201315] ; Anhui Provincial Natural Science Foundation[2008085MB49] ; Open Project Fund of the Key Laboratory of the Ministry of Education[JKZD20202] ; Funded Project of Anhui Medical Universitys Research Level Improvement Program[2021xkjT004] ; Anhui Medical University Basic and Clinical Cooperative Research Promotion Program[2021xkjT041]

Science & Technology - Other Topics ; Pharmacology & Pharmacy

Nanoscience & Nanotechnology ; Pharmacology & Pharmacy

WOS:000961975400001

DOVE MEDICAL PRESS LTD

2-s2.0-85151795401

Scopus

1.Department of Pathophysiology,School of Basic Medical Science,Anhui Medical University,Hefei,230032,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
3.Institute of Clinical Laboratory Science,Jinling Hospital,School of Medicine,Nanjing University,Nanjing,210002,China
4.Institute of Technical Biology & Agriculture Engineering,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei,230032,China
5.Department of Medical Laboratory,Luoyang Orthopedic Hospital of Henan Province,Orthopedic Institute of Henan Province,Luoyang,China
6.School of Medical Science,Huang He Science and Technology University,Zhengzhou,China
7.School of Biological Sciences,Nanyang Technological University,639798,Singapore
8.Department of Physiology and Biophysics,Miller School of Medicine,University of Miami,Miami,33136,United States

Wang，Meimei,Zhang，Zhenzhen,Sun，Ning,et al. Gold Nanoparticles Reduce Food Sensation in Caenorhabditis elegans via the Voltage-Gated Channel EGL-19[J]. International journal of nanomedicine,2023,18:1659-1676.

Wang，Meimei.,Zhang，Zhenzhen.,Sun，Ning.,Yang，Baolin.,Mo，Jihao.,...&Wang，Lei.(2023).Gold Nanoparticles Reduce Food Sensation in Caenorhabditis elegans via the Voltage-Gated Channel EGL-19.International journal of nanomedicine,18,1659-1676.

Wang，Meimei,et al."Gold Nanoparticles Reduce Food Sensation in Caenorhabditis elegans via the Voltage-Gated Channel EGL-19".International journal of nanomedicine 18(2023):1659-1676.