MELLT3 protects against cerebral ischemia-reperfusion (I/R) injury through up-regulation of m6A modification

Jin，Jing1; Wang，Xinghua2; Zheng，Xiaoxiao3; Lan，Jiahua3; Zheng，Li3; Cai，Ying3; Chen，Hui4; Wang，Hongwei5; Zheng，Lifang6

2023

10.32604/biocell.2023.026016

Biocell   Impact Factor & Quartile

0327-9545

1667-5746

Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury (CIRI). The exact mechanisms underlying I/R injury are unclear. In this study, we aimed to determine the role of m6A-modified methylase complex methyltransferase-like 3 (METTL3) in cerebral ischemiareperfusion (I/R) injury. We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytescerebellar (MA-C) and the brain of middle cerebral artery occlusion (MCAO) model mice. METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation, which increased with METTL3 over-expression. Flow cytometry analysis showed that silencing METTL3 significantly enhanced OGD/RX-induced MAC apoptosis, which was significantly reduced with METTL3 up-regulation. In an MCAO model, METTL3 overexpression significantly reduced cerebral infarction area and decreased brain cell apoptosis, indicating that METTL3 OE treatment could ameliorate brain edema and injury. Thus, METTL3 could be used as a target to treat I/R injury.

Cerebral ischemia-reperfusion m6A METTL3

SCI

English

Corresponding

WOS:000950611400006

BIOLOGY & BIOCHEMISTRY

2-s2.0-85150202619

Scopus

1.Department of Neurosurgery,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,310003,China
2.Department of Neurology,Puren Hospital Affiliated,Wuhan University of Science and Technology,Wuhan,430081,China
3.Cancer Institute of Integrated Traditional Chinese and Western Medicine,Zhejiang Academy of Traditional Chinese Medicine,Tongde Hospital of Zhejiang Province,Hangzhou,310012,China
4.Department of Animal Experimental Center,The First Affiliated Hospital,Zhejiang University,School of Medicine,Hangzhou,10003,China
5.Department of Anesthesiology,Tongde Hospital of Zhejiang Province,Hangzhou,310012,China
6.Department of Neurology,Yantian Hospital of Southern University of Science and Technology,Shenzhen,518000,China

Jin，Jing,Wang，Xinghua,Zheng，Xiaoxiao,et al. MELLT3 protects against cerebral ischemia-reperfusion (I/R) injury through up-regulation of m6A modification[J]. Biocell,2023,47(3):619-626.

Jin，Jing.,Wang，Xinghua.,Zheng，Xiaoxiao.,Lan，Jiahua.,Zheng，Li.,...&Zheng，Lifang.(2023).MELLT3 protects against cerebral ischemia-reperfusion (I/R) injury through up-regulation of m6A modification.Biocell,47(3),619-626.

Jin，Jing,et al."MELLT3 protects against cerebral ischemia-reperfusion (I/R) injury through up-regulation of m6A modification".Biocell 47.3(2023):619-626.