Detecting latent tuberculosis infection with a breath test using mass spectrometer: A pilot cross-sectional study

Fu，Liang1; Feng，Yong2; Ren，Tantan1; Yang，Min1; Yang，Qianting3; Lin，Yi1; Zeng，Hui4; Zhang，Jiaohong5; Liu，Lei1; Li，Qingyun2; He，Mengqi2; Zhang，Peize1; Chen，Haibin2; Deng，Guofang1

2023

10.5582/bst.2022.01476

BioScience Trends   Impact Factor & Quartile

1881-7815

1881-7823

Mycobacterium tuberculosis (M.tb) infects a quarter of the world's population and may progress to active tuberculosis (ATB). There is no gold standard for diagnosing latent tuberculosis infection (LTBI). Some immunodiagnostic tests are recommended to detect LTBI but can not distinguish ATB from LTBI. The breath test is useful for diagnosing ATB compared to healthy subjects but was never studied for LTBI. This proof-of-concept study (Chinese Clinical Trials Registry number: ChiCTR2200058346) was the first to explore a novel, rapid, and simple LTBI detection method via breath test on high-pressure photon ionization time-of-flight mass spectrometry (HPPI-TOFMS). The case group of LTBI subjects (n = 185) and the control group (n = 250), which included ATB subgroup (n = 121) and healthy control (HC) subgroup (n = 129), were enrolled. The LTBI detection model indicated that a breath test via HPPI-TOFMS could distinguish LTBI from the control with a sensitivity of 80.0% (95% CI: 67.6%, 92.4%) and a specificity of 80.8% (95% CI: 71.8%, 89.9%). Nevertheless, further intensive studies with a larger sample size are required for clinical application.

breath diagnosis latent tuberculosis infection tuberculosis volatile organic compounds

SCI

English

First ; Corresponding

WOS:000908367100001

2-s2.0-85150079422

Scopus

1.Division Two of Pulmonary Diseases Department,The Third People's Hospital of Shenzhen,National clinical research center for infectious disease,Southern University of Science and Technology,Shenzhen,Guangdong,China
2.Breax Laboratory,PCAB Research Center of Breath and Metabolism,Beijing,China
3.Guangdong Key Lab for Diagnosis & Treatment of Emerging Infectious Disease,The Third People's Hospital of Shenzhen,National clinical research center for infectious disease,Southern University of Science and Technology,Shenzhen,Guangdong,China
4.Medical Examination Department,The Third People's Hospital of Shenzhen,National clinical research center for infectious disease,Southern University of Science and Technology,Shenzhen,Guangdong,China
5.Pulmonary Diseases Out-patient Department,The Third People's Hospital of Shenzhen,National clinical research center for infectious disease,Southern University of Science and Technology,Shenzhen,Guangdong,China

Fu，Liang,Feng，Yong,Ren，Tantan,et al. Detecting latent tuberculosis infection with a breath test using mass spectrometer: A pilot cross-sectional study[J]. BioScience Trends,2023,17(1):73-77.

Fu，Liang.,Feng，Yong.,Ren，Tantan.,Yang，Min.,Yang，Qianting.,...&Deng，Guofang.(2023).Detecting latent tuberculosis infection with a breath test using mass spectrometer: A pilot cross-sectional study.BioScience Trends,17(1),73-77.

Fu，Liang,et al."Detecting latent tuberculosis infection with a breath test using mass spectrometer: A pilot cross-sectional study".BioScience Trends 17.1(2023):73-77.