中文版 | English
Title

一株新海洋粘质沙雷氏菌产灵菌红素发酵条件优化

Alternative Title
Fermentation optimization of a new prodigiosin-producing marine Serratia marcescens strain
Author
Publication Years
2022
DOI
Source Title
ISSN
1006-8376
Volume44Issue:6Pages:540-552
Abstract
灵菌红素是由微生物产生的一种红色次级代谢产物,因其具有抗菌、抗癌和抗疟疾等功效,受到了生物医药领域的广泛关注.微生物发酵是当前产灵菌红素的主要方法,分离筛选高产灵菌红素的微生物、优化发酵条件是提高灵菌红素产率的重要途径.本研究从深圳湾筛选出一株含有红色色素的菌株,并基于16SrRNA基因序列对该菌株进行了系统发育分析和物种鉴定.紫外可见光全波长扫描和HPLC-MS图谱分析证明,该菌株所产红色色素为灵菌红素.进一步通过单因素试验和正交优化法优化了该菌产灵菌红素的发酵条件和发酵培养基组分.系统发育分析表明,该菌株为一株海洋粘质沙雷氏菌(Serratia marcescens),并将其命名为S.marcescens SOCE 001.产灵菌红素的最佳发酵条件为:温度28℃、振荡培养转速220 r/min、培养基pH7.发酵培养基最佳组合为:果糖添加量2 g/L、蛋白胨添加量10 g/L,MgSO4添加量2 g/L.优化发酵条件后,经24h培养,灵菌红素产量可达2.468 g/L.
Keywords
URL[Source Record]
Language
Chinese
SUSTech Authorship
Others
Funding Project
91851210:国家自然科学基金 ; 2021B1515120080:广东省基础与应用基础研究基金 ; MELRS2210:近海海洋环境科学国家重点实验室(厦门大学)访问学者与开放课题 ; SJZLGC202111:前沿海洋技术三维一体环境监测实验教学示范中心
Data Source
WanFang
WanFangID
ajshswzy202206003
Citation statistics
Cited Times [WOS]:0
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/525011
DepartmentDepartment of Ocean Science and Engineering
Affiliation
1.南方科技大学海洋科学与工程系,广东深圳 518055
2.南方科技大学深圳海洋地球古菌组学重点实验室,广东深圳518055
3.南方海洋科学与工程广东省实验室(广州),广东广州511458
Recommended Citation
GB/T 7714
郭静,徐帅帅,王椅冬,等. 一株新海洋粘质沙雷氏菌产灵菌红素发酵条件优化[J]. 生物资源,2022,44(6):540-552.
APA
郭静,徐帅帅,王椅冬,张传伦,&侯圣伟.(2022).一株新海洋粘质沙雷氏菌产灵菌红素发酵条件优化.生物资源,44(6),540-552.
MLA
郭静,et al."一株新海洋粘质沙雷氏菌产灵菌红素发酵条件优化".生物资源 44.6(2022):540-552.
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