Distinct chromatin signatures in the Arabidopsis male gametophyte

Danling,Zhu1; Yi,Wen2; Wanyue,Yao1; Haiyan,Zheng3; Sixian,Zhou1; Qiqi,Zhang1; Li-Jia,Qu4; Xi,Chen1; Zhe,Wu1

2023-03-01

10.1038/s41588-023-01329-7

NATURE GENETICS   Impact Factor & Quartile

1061-4036

1546-1718

Epigenetic reprogramming in the germline contributes to the erasure of epigenetic inheritance across generations in mammals but remains poorly characterized in plants. Here we profiled histone modifications throughout Arabidopsis male germline development. We find that the sperm cell has widespread apparent chromatin bivalency, which is established by the acquisition of H3K27me3 or H3K4me3 at pre-existing H3K4me3 or H3K27me3 regions, respectively. These bivalent domains are associated with a distinct transcriptional status. Somatic H3K27me3 is generally reduced in sperm, while dramatic loss of H3K27me3 is observed at only similar to 700 developmental genes. The incorporation of the histone variant H3.10 facilitates the establishment of sperm chromatin identity without a strong impact on resetting of somatic H3K27me3. Vegetative nuclei harbor thousands of specific H3K27me3 domains at repressed genes, while pollination-related genes are highly expressed and marked by gene body H3K4me3. Our work highlights putative chromatin bivalency and restricted resetting of H3K27me3 at developmental regulators as key features in plant pluripotent sperm.

SCI

English

First ; 共同第一 ; Corresponding

National Natural Science Foundation of China["32270369","31970277","32170348","2022A1515010505"] ; null[32070645] ; null[2016ZT06S172] ; null[JCYJ20210324104803009] ; null[JCYJ20190809141201671] ; null[20200925153455004] ; null[KYTDPT20181011104005] ; null[ZDSYS20200811144002008] ; null[2019KSYS006]

Genetics & Heredity

Genetics & Heredity

WOS:000971784400004

NATURE PORTFOLIO

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.
2.Shenzhen Key Laboratory of Gene Regulation and Systems Biology, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China
3.Center for Advanced Biotechnology and Medicine, Biological Mass Spectrometry Facility, Rutgers University, Piscataway, NJ, USA
4.State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, College of Life Sciences, Peking University, Beijing, China

Danling,Zhu,Yi,Wen,Wanyue,Yao,et al. Distinct chromatin signatures in the Arabidopsis male gametophyte[J]. NATURE GENETICS,2023.

Danling,Zhu.,Yi,Wen.,Wanyue,Yao.,Haiyan,Zheng.,Sixian,Zhou.,...&Zhe,Wu.(2023).Distinct chromatin signatures in the Arabidopsis male gametophyte.NATURE GENETICS.

Danling,Zhu,et al."Distinct chromatin signatures in the Arabidopsis male gametophyte".NATURE GENETICS (2023).