中文版 | English
Title

Uniform thin ice on ultraflat graphene for high-resolution cryo-EM

Author
Corresponding AuthorLiu, Nan; Wei, Xiaoding; Wang, Hong-Wei; Peng, Hailin
Publication Years
2023-01-05
DOI
Source Title
ISSN
1548-7091
EISSN
1548-7105
Volume20Issue:1
Abstract

Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, the homogeneity of ice thickness, a key factor to ensure high image quality, is poorly controlled during specimen preparation and has become one of the main challenges for high-resolution cryo-EM. Here we found that the uniformity of thin ice relies on the surface flatness of the supporting film, and developed a method to use ultraflat graphene (UFG) as the support for cryo-EM specimen preparation to achieve better control of vitreous ice thickness. We show that the uniform thin ice on UFG improves the image quality of vitrified specimens. Using such a method we successfully determined the three-dimensional structures of hemoglobin (64 kDa), alpha-fetoprotein (67 kDa) with no symmetry, and streptavidin (52 kDa) at a resolution of 3.5 angstrom, 2.6 angstrom and 2.2 angstrom, respectively. Furthermore, our results demonstrate the potential of UFG for the fields of cryo-electron tomography and structure-based drug discovery.

URL[Source Record]
Indexed By
Language
English
Important Publications
NI Journal Papers
SUSTech Authorship
Others
Funding Project
National Natural Science Foundation of China[
WOS Research Area
Biochemistry & Molecular Biology
WOS Subject
Biochemical Research Methods
WOS Accession No
WOS:000949616100016
Publisher
ESI Research Field
BIOLOGY & BIOCHEMISTRY
Data Source
Web of Science
Citation statistics
Cited Times [WOS]:1
Document TypeJournal Article
Identifierhttp://kc.sustech.edu.cn/handle/2SGJ60CL/527710
DepartmentDepartment of Biology
生命科学学院
Affiliation
1.Peking Univ, Ctr Nanochem, Beijing Sci & Engn Ctr Nanocarbons, Beijing Natl Lab Mol Sci,Coll Chem & Mol Engn, Beijing, Peoples R China
2.Beijing Graphene Inst BGI, Beijing, Peoples R China
3.Tsinghua Univ, Minist Educ Key Lab Prot Sci, Beijing Frontier Res Ctr Biol Struct, Beijing Adv Innovat Ctr Struct Biol,Sch Life Sci, Beijing, Peoples R China
4.Peking Univ, Coll Engn, Dept Mech & Engn Sci, State Key Lab Turbulence & Complex Syst, Beijing, Peoples R China
5.Hainan Med Coll, Hainan Prov Key Lab Carcinogenesis & Intervent, Haikou, Peoples R China
6.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen, Peoples R China
7.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing, Peoples R China
8.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Beijing, Peoples R China
9.Peking Univ, Beijing Innovat Ctr Engn Sci & Adv Technol, Beijing, Peoples R China
10.Peking Univ, Nanchang Innovat Inst, Nanchang, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Liming,Liu, Nan,Gao, Xiaoyin,et al. Uniform thin ice on ultraflat graphene for high-resolution cryo-EM[J]. NATURE METHODS,2023,20(1).
APA
Zheng, Liming.,Liu, Nan.,Gao, Xiaoyin.,Zhu, Wenqing.,Liu, Kun.,...&Peng, Hailin.(2023).Uniform thin ice on ultraflat graphene for high-resolution cryo-EM.NATURE METHODS,20(1).
MLA
Zheng, Liming,et al."Uniform thin ice on ultraflat graphene for high-resolution cryo-EM".NATURE METHODS 20.1(2023).
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