Title | The RNA-binding protein hnRNP F is required for the germinal center B cell response |
Author | |
Corresponding Author | Xu, Shengli; Ou, Xijun |
Publication Years | 2023-03-30
|
DOI | |
Source Title | |
EISSN | 2041-1723
|
Volume | 14Issue:1 |
Abstract | ["The germinal centre (GC) response is characterized by regulated production of high affinity, class-switched antibodies in response to T-cell dependent antigens. Here authors show that the GC response is not only regulated at the transcriptional and protein levels, but also by the RNA-binding protein hnRNP F via alternative splicing of the co-stimulatory molecule CD40.","The T cell-dependent (TD) antibody response involves the generation of high affinity, immunoglobulin heavy chain class-switched antibodies that are generated through germinal center (GC) response. This process is controlled by coordinated transcriptional and post-transcriptional gene regulatory mechanisms. RNA-binding proteins (RBPs) have emerged as critical players in post-transcriptional gene regulation. Here we demonstrate that B cell-specific deletion of RBP hnRNP F leads to diminished production of class-switched antibodies with high affinities in response to a TD antigen challenge. B cells deficient in hnRNP F are characterized by defective proliferation and c-Myc upregulation upon antigenic stimulation. Mechanistically, hnRNP F directly binds to the G-tracts of Cd40 pre-mRNA to promote the inclusion of Cd40 exon 6 that encodes its transmembrane domain, thus enabling appropriate CD40 cell surface expression. Furthermore, we find that hnRNP A1 and A2B1 can bind to the same region of Cd40 pre-mRNA but suppress exon 6 inclusion, suggesting that these hnRNPs and hnRNP F might antagonize each-other's effects on Cd40 splicing. In summary, our study uncovers an important posttranscriptional mechanism regulating the GC response."] |
URL | [Source Record] |
Indexed By | |
Language | English
|
Important Publications | NI Journal Papers
|
SUSTech Authorship | First
; Corresponding
|
Funding Project | Shenzhen Science and Technology Innovation Commission[
|
WOS Research Area | Science & Technology - Other Topics
|
WOS Subject | Multidisciplinary Sciences
|
WOS Accession No | WOS:000961133600029
|
Publisher | |
Data Source | Web of Science
|
Citation statistics |
Cited Times [WOS]:0
|
Document Type | Journal Article |
Identifier | http://kc.sustech.edu.cn/handle/2SGJ60CL/527711 |
Department | School of Life Sciences 工学院 工学院_计算机科学与工程系 |
Affiliation | 1.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Peoples R China 2.Taizhou Univ, Med Sch, Taizhou 318000, Peoples R China 3.Southern Univ Sci & Technol, Coll Engn, Dept Comp Sci & Engn, Shenzhen 518055, Peoples R China 4.Agcy Sci Technol & Res, Singapore Immunol Network, Singapore 138648, Singapore 5.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore 117599, Singapore |
First Author Affilication | School of Life Sciences |
Corresponding Author Affilication | School of Life Sciences |
First Author's First Affilication | School of Life Sciences |
Recommended Citation GB/T 7714 |
Huang, Hengjun,Li, Yuxing,Zhang, Gaopu,et al. The RNA-binding protein hnRNP F is required for the germinal center B cell response[J]. NATURE COMMUNICATIONS,2023,14(1).
|
APA |
Huang, Hengjun.,Li, Yuxing.,Zhang, Gaopu.,Ruan, Gui-Xin.,Zhu, Zhijian.,...&Ou, Xijun.(2023).The RNA-binding protein hnRNP F is required for the germinal center B cell response.NATURE COMMUNICATIONS,14(1).
|
MLA |
Huang, Hengjun,et al."The RNA-binding protein hnRNP F is required for the germinal center B cell response".NATURE COMMUNICATIONS 14.1(2023).
|
Files in This Item: | There are no files associated with this item. |
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment