Integrated mass spectrometry strategy for functional protein complex discovery and structural characterization

Liu，Zheyi1,3; Chen，Xiong2; Yang，Shirui1,3; Tian，Ruijun2; Wang，Fangjun1,3

2023-06-01

10.1016/j.cbpa.2023.102305

Current Opinion in Chemical Biology   Impact Factor & Quartile

1367-5931

1879-0402

The discovery of functional protein complex and the interrogation of the complex structure-function relationship (SFR) play crucial roles in the understanding and intervention of biological processes. Affinity purification-mass spectrometry (AP-MS) has been proved as a powerful tool in the discovery of protein complexes. However, validation of these novel protein complexes as well as elucidation of their molecular interaction mechanisms are still challenging. Recently, native top-down MS (nTDMS) is rapidly developed for the structural analysis of protein complexes. In this review, we discuss the integration of AP-MS and nTDMS in the discovery and structural characterization of functional protein complexes. Further, we think the emerging artificial intelligence (AI)-based protein structure prediction is highly complementary to nTDMS and can promote each other. We expect the hybridization of integrated structural MS with AI prediction to be a powerful workflow in the discovery and SFR investigation of functional protein complexes.

Affinity purification-mass spectrometry Artificial intelligence prediction Native top-down mass spectrometry Protein complex Structure-function relationship

English

Others

BIOLOGY & BIOCHEMISTRY

2-s2.0-85152381648

Scopus

1.CAS Key Laboratory of Separation Sciences for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China
2.Department of Chemistry,College of Science,Southern University of Science and Technology,Shenzhen,518055,China
3.University of Chinese Academy of Sciences,Beijing,100049,China

Liu，Zheyi,Chen，Xiong,Yang，Shirui,et al. Integrated mass spectrometry strategy for functional protein complex discovery and structural characterization[J]. Current Opinion in Chemical Biology,2023,74.

Liu，Zheyi,Chen，Xiong,Yang，Shirui,Tian，Ruijun,&Wang，Fangjun.(2023).Integrated mass spectrometry strategy for functional protein complex discovery and structural characterization.Current Opinion in Chemical Biology,74.

Liu，Zheyi,et al."Integrated mass spectrometry strategy for functional protein complex discovery and structural characterization".Current Opinion in Chemical Biology 74(2023).