南方科技大学知识苑(SUSTech KC): 一种免扩增的核酸检测方法及其应用

Title	一种免扩增的核酸检测方法及其应用
Alternative Title	Amplification-free nucleic acid detection method and application thereof
Author	蒋兴宇; 王斗
First Inventor	蒋兴宇
Original applicant	南方科技大学
First applicant	南方科技大学
Address of First applicant	518055 广东省深圳市南山区桃源街道学苑大道1088号
Current applicant	南方科技大学
Address of Current applicant	518055 广东省深圳市南山区桃源街道学苑大道1088号 (广东,深圳,南山区)
First Current Applicant	南方科技大学
Address of First Current Applicant	518055 广东省深圳市南山区桃源街道学苑大道1088号 (广东,深圳,南山区)
Application Number	CN202210180046.0
Application Date	2022-02-25
Open (Notice) Number	CN114540547A
Date Available	2022-05-27
Status of Patent	实质审查
Legal Date	2022-06-14
Subtype	发明申请
SUSTech Authorship	First
Abstract	本发明属于核酸检测领域，公开了一种免扩增的核酸检测方法及其应用。本发明的核酸检测方法包括：用捕获探针捕获待检核酸，形成捕获探针‑待检核酸复合物；将捕获探针‑待检核酸复合物与CRISPR反应体系混合形成反应液，将反应液通入微流控芯片的微孔中进行反应；对产生的荧光进行显微观察，以确定核酸检测结果。本发明将CRISPR技术与微流控技术相结合开发的核酸检测方法，不需要核酸扩增就可以实现核酸的单分子检测，简便快速，不会造成扩增核酸时引起的气溶胶污染，灵敏度高，可检测到阿摩尔级别的核酸分子，极大地扩大了使用范围。
Other Abstract	The invention belongs to the field of nucleic acid detection, and discloses an amplification-free nucleic acid detection method and application thereof. The nucleic acid detection method comprises the following steps: capturing to-be-detected nucleic acid by using a capture probe to form a capture probe-to-be-detected nucleic acid compound; mixing the capture probe-to-be-detected nucleic acid compound with a CRISPR reaction system to form reaction liquid, and introducing the reaction liquid into micropores of a micro-fluidic chip for reaction; and carrying out microscopic observation on the generated fluorescence to determine a nucleic acid detection result. According to the nucleic acid detection method developed by combining the CRISPR technology and the microfluidic technology, single molecule detection of nucleic acid can be realized without nucleic acid amplification, the method is simple, convenient and rapid, aerosol pollution caused by nucleic acid amplification is avoided, the sensitivity is high, nucleic acid molecules at the almole level can be detected, and the application range is greatly expanded.
CPC Classification Number	C12Q1/701 ; C12Q1/6837 ; C12Q2521/327 ; C12Q2525/161 ; C12Q2563/107 ; C12Q2565/629 ; Y02A50/30
IPC Classification Number	C12Q1/70 ; C12Q1/6837 ; C12R1/93
INPADOC Legal Status	（ENTRY INTO FORCE OF REQUEST FOR SUBSTANTIVE EXAMINATION）[2022-06-14][CN]
INPADOC Patent Family Count	1
Extended Patent Family Count	1
Priority date	2022-02-25
Patent Agent	刘燚
Agency	广州嘉权专利商标事务所有限公司
URL	[Source Record]
Data Source	PatSnap
Document Type	Patent
Identifier	http://kc.sustech.edu.cn/handle/2SGJ60CL/531622
Department	Department of Biomedical Engineering 前沿与交叉科学研究院
Recommended Citation GB/T 7714	蒋兴宇,王斗. 一种免扩增的核酸检测方法及其应用.

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