南方科技大学知识苑(SUSTech KC): 一种快速制备高通量测序文库的方法及应用

Title	一种快速制备高通量测序文库的方法及应用
Alternative Title	Method for rapid preparation of high-throughput sequencing library and application
Author	贺建奎
First Inventor	贺建奎
Original applicant	南方科技大学
First applicant	南方科技大学
Address of First applicant	518000 广东省深圳市南山区西丽学苑大道1088号
Current applicant	南方科技大学
Address of Current applicant	518000 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
First Current Applicant	南方科技大学
Address of First Current Applicant	518000 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
Application Number	CN201510107817.3
Application Date	2015-03-11
Open (Notice) Number	CN104789552A
Date Available	2015-07-22
Status of Patent	驳回
Legal Date	2019-06-07
Subtype	发明申请
SUSTech Authorship	First
Abstract	本发明提供了一种快速制备高通量测序文库的方法及应用。本发明提供的技术方案基于我们对该技术流程的深入研究，对加adapter的反应体系进行了优化，提高了加adapter的效率，降低了文库构建成本及人为操作误差出现的可能性。此外，我们还选择了最优的PCR反应体系，利用有些酶(如Taq酶)加A的特性，达到模版扩增的同时对PCR产物末端加A的效果，相比当前将模板扩增与加A步骤分开进行的方法，本发明大大的减少了整个构库时间。
Other Abstract	The invention provides a method for rapid preparation of a high-throughput sequencing library and application. The technical scheme provided by the invention is based on the in-depth study of the technical process, optimizes an adapter added reaction system, improves the adapter adding efficiency, and reduces the library construction cost and possibility of artificial operation error. In addition, the invention selects an optimal PCR reaction system, and utilizes the A adding characteristic of some enzymes (like Taq enzyme) to reach the effects of template amplification and adding of A to a PCR product end simultaneously. Compared with the current method of conducting template amplification and A adding separately, the method provided by the invention greatly reduces the whole library construction time.
IPC Classification Number	C12N15/10 ; C12Q1/68 ; C40B50/06
INPADOC Legal Status	（-REJECTION OF INVENTION PATENT APPLICATION AFTER PUBLICATION）[2019-06-07][CN]
INPADOC Patent Family Count	1
Extended Patent Family Count	1
Priority date	2015-03-11
Patent Agent	郝传鑫 ; 熊永强
Agency	广州三环专利代理有限公司
URL	[Source Record]
Data Source	PatSnap
Document Type	Patent
Identifier	http://kc.sustech.edu.cn/handle/2SGJ60CL/531852
Department	Department of Biology
Recommended Citation GB/T 7714	贺建奎. 一种快速制备高通量测序文库的方法及应用.

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