南方科技大学知识苑(SUSTech KC): 检测古菌细胞膜脂中带完整极性头化合物的方法

Title	检测古菌细胞膜脂中带完整极性头化合物的方法
Alternative Title	Method of detecting compound with intact polar head in archaea cell membrane lipid
Author	张传伦; 陈雨霏; 郑峰峰
First Inventor	张传伦
Original applicant	南方科技大学
First applicant	南方科技大学
Address of First applicant	518055 广东省深圳市南山区西丽大学城学苑大道1088号
Current applicant	南方科技大学
Address of Current applicant	518055 广东省深圳市南山区西丽大学城学苑大道1088号 (广东,深圳,南山区)
First Current Applicant	南方科技大学
Address of First Current Applicant	518055 广东省深圳市南山区西丽大学城学苑大道1088号 (广东,深圳,南山区)
Application Number	CN201810342391.3
Application Date	2018-04-17
Open (Notice) Number	CN110057926A
Date Available	2019-07-26
Status of Patent	驳回
Legal Date	2023-03-03
Subtype	发明申请
SUSTech Authorship	First
Abstract	本发明提供一种检测古菌细胞膜脂中带完整极性头化合物的方法，包括以下步骤：在MRM下将色谱柱温度设定为40～50℃，流速为0.15～0.25mL/min；控制离子源干燥气流速2.5～3.5L/min，温度280～320℃；鞘气流速8.5～9.5L/min，温度280～320℃；喷雾压力400～450kPa；毛细管电压3.5～5.0kV；喷射电压650～750V；碎裂反应的电压150～250仪器单位，碰撞能量10～50仪器单位；不带任何极性头的核心脂部分质荷比为1302.3～1292.3；进行古菌细胞膜脂中带完整极性头化合物的检测。本发明具有检测灵敏度高、检出限低的特点。
Other Abstract	The invention provides a method of detecting a compound with an intact polar head in archaea cell membrane lipid. The method comprises the steps that under MRM, chromatographic column temperature is set to be 40-50 DEG C, and the flow velocity is set to be 0.15-0.25mL/min; the flow velocity of an ion source drying gas is controlled to be 2.5-3.5L/min, and the temperature of the ion source drying gas is controlled to be 280-320 DEG C; the flow velocity of a sheath gas is controlled to be 8.5-9.5L/min, and the temperature of the sheath gas is controlled to be 280-320 DEG C; spray pressure is controlled to be 400-450kPa; the capillary voltage is controlled to be 3.5-5.0kV; the jet voltage is controlled to be 650-750V; the voltage of a fragmentation reaction is controlled to be 150-250 instrument units, and collision energy is controlled to be 10-50 instrument units; the mass-to-charge ratio of a core lipid part without any polar head is 1,302.3-1,292.3; and detection of the compound withthe intact polar head in the archaea cell membrane lipid is performed. The method has the advantages of being high in detection sensitivity and low in detection limit.
CPC Classification Number	G01N30/02
IPC Classification Number	G01N30/02
INPADOC Legal Status	（-REJECTION OF INVENTION PATENT APPLICATION AFTER PUBLICATION）[2023-03-03][CN]
INPADOC Patent Family Count	1
Extended Patent Family Count	1
Priority date	2018-04-17
Patent Agent	官建红
Agency	深圳中一专利商标事务所
URL	[Source Record]
Data Source	PatSnap
Document Type	Patent
Identifier	http://kc.sustech.edu.cn/handle/2SGJ60CL/531860
Department	Department of Ocean Science and Engineering
Recommended Citation GB/T 7714	张传伦,陈雨霏,郑峰峰. 检测古菌细胞膜脂中带完整极性头化合物的方法.

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