南方科技大学知识苑(SUSTech KC): 探针及其制备方法和应用

Title	探针及其制备方法和应用
Alternative Title	Probe, and preparation method and application thereof
Author	卢杰
First Inventor	卢杰
Original applicant	南方科技大学
First applicant	南方科技大学
Address of First applicant	518055 广东省深圳市南山区西丽学苑大道1088号
Current applicant	南方科技大学
Address of Current applicant	518055 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
First Current Applicant	南方科技大学
Address of First Current Applicant	518055 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
Application Number	CN202011134712.4
Application Date	2020-10-21
Open (Notice) Number	CN112111564A
Date Available	2020-12-22
Status of Patent	实质审查
Legal Date	2021-01-08
Subtype	发明申请
SUSTech Authorship	First
Abstract	本发明提供一种探针及其制备方法和应用。该探针包括核酸片段，核酸片段通过多臂修饰性PEG标记有荧光物质。核苷酸通过多臂修饰性PEG实现PEG化，多臂修饰性PEG的修饰基团与荧光物质发生偶联反应从而使荧光物质标记到核酸片段上。以此形成的探针中，单个核苷酸有多个修饰基团形成的偶联位点，更多的偶联位点可以结合更多的荧光物质。因而，标记后的探针其单位长度上的荧光物质更多，检测时表现出的荧光强度更高，从而能够有效提高检测的灵敏度和精确性。
Other Abstract	The invention provides a probe, and a preparation method and application thereof. The probe comprises a nucleic acid fragment, and the nucleic acid fragment is labeled with a fluorescent substance through multi-arm modified PEG. Nucleotide realizes PEGylation through the multi-arm modified PEG, and a modification group of the multi-arm modified PEG and the fluorescent substance undergo a couplingreaction so as to allow the fluorescent substance to be labeled on the nucleic acid fragment. In the probe formed in the invention, a single nucleotide has coupling sites formed by a plurality of modification groups, and more coupling sites can be combined with more fluorescent substances. Thus, the labeled probe has more fluorescent substances in unit length and shows higher fluorescence intensity during detection, so detection sensitivity and accuracy can be effectively improved.
CPC Classification Number	C12Q1/6841 ; C12Q2563/107
IPC Classification Number	C12Q1/6841 ; C12Q1/6806 ; C12N15/11
INPADOC Legal Status	（ENTRY INTO FORCE OF REQUEST FOR SUBSTANTIVE EXAMINATION）[2021-01-08][CN]
INPADOC Patent Family Count	1
Extended Patent Family Count	1
Priority country	CN
Priority number	202010758024.9
Priority date	2020-07-31
Patent Agent	刘燚
Agency	广州嘉权专利商标事务所有限公司
URL	[Source Record]
Data Source	PatSnap
Document Type	Patent
Identifier	http://kc.sustech.edu.cn/handle/2SGJ60CL/531998
Department	Department of Biomedical Engineering
Recommended Citation GB/T 7714	卢杰. 探针及其制备方法和应用.

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