南方科技大学知识苑(SUSTech KC): 一种血清多肽组学的质谱检测方法

Title	一种血清多肽组学的质谱检测方法
Alternative Title	Mass spectrometry detecting method for serum polypeptides
Author	林琳
First Inventor	林琳
Original applicant	南方科技大学
First applicant	南方科技大学
Address of First applicant	518000 广东省深圳市南山区西丽学苑大道1088号
Current applicant	南方科技大学
Address of Current applicant	518000 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
First Current Applicant	南方科技大学
Address of First Current Applicant	518000 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
Application Number	CN201811642365.9
Application Date	2018-12-29
Open (Notice) Number	CN109725078A
Date Available	2019-05-07
Status of Patent	驳回
Legal Date	2022-12-02
Subtype	发明申请
SUSTech Authorship	First
Abstract	本发明提供了一种血清多肽组学的质谱检测方法，包括样本制备、数据非依赖性质谱采集及数据处理；该方法针对血清多肽组学样本发展了数据非依赖性质谱采集方法，通过对血清样本的预分析实验获取样本的母离子分布情况，由此确定最优的可变隔离窗口；通过对多肽组学样品制备方法、质谱采集方法和数据处理方法的优化，各步骤各条件相互配合，最终实现多肽检测数目、鉴定重现性和定量准确性的显著提高；本发明方法具有高通量、高重现性和高准确性的特点，适合于大规模临床血清样本的分析及疾病标志物的检测，具有广泛的应用前景和巨大的市场价值。
Other Abstract	The invention provides a mass spectrometry detecting method for a serum polypeptides, comprising sample preparation, data-independent mass spectrometry acquisition, and data processing. According to the mass spectrometry detecting method for the serum polypeptides, a data-independent mass spectrometry acquisition method for serum peptidomics samples is developed, and the parent ion distribution ofthe sample by pre-analytical experiment on serum samples is obtained, thereby obtaining an optimal variable isolation window. Each condition and each step cooperate with each other by optimizing thepreparation method of the polypeptideomics sample, the mass spectrometry acquisition method, and the data processing method, and finally, the number of the detection of the polypeptide, the reproducibility of the identification, and the quantitative accuracy are significantly improved. The method of the invention has the characteristics of high throughput, high reproducibility, and high accuracy,and is suitable for analysis of large-scale clinical serum samples and detection of disease markers, thereby having wide application prospects and great market value.
IPC Classification Number	G01N30/02 ; G01N30/06
INPADOC Legal Status	（-REJECTION OF INVENTION PATENT APPLICATION AFTER PUBLICATION）[2022-12-02][CN]
INPADOC Patent Family Count	1
Extended Patent Family Count	1
Priority date	2018-12-29
Patent Agent	巩克栋
Agency	北京品源专利代理有限公司
URL	[Source Record]
Data Source	PatSnap
Document Type	Patent
Identifier	http://kc.sustech.edu.cn/handle/2SGJ60CL/534126
Department	Southern University of Science and Technology
Recommended Citation GB/T 7714	林琳. 一种血清多肽组学的质谱检测方法.

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