南方科技大学知识苑(SUSTech KC): 爪蛙嵌合体及其制备方法

Title	爪蛙嵌合体及其制备方法
Alternative Title	Clawed frog chimera and preparation method thereof
Author	陈永龙; 李兰心 ; 石照应
First Inventor	陈永龙
Original applicant	南方科技大学
First applicant	南方科技大学
Address of First applicant	518055 广东省深圳市南山区西丽学苑大道1088号
Current applicant	南方科技大学
Address of Current applicant	518055 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
First Current Applicant	南方科技大学
Address of First Current Applicant	518055 广东省深圳市南山区西丽学苑大道1088号 (广东,深圳,南山区)
Application Number	CN202010928618.X
Application Date	2020-09-07
Open (Notice) Number	CN112175994A
Date Available	2021-01-05
Status of Patent	实质审查
Legal Date	2021-01-22
Subtype	发明申请
SUSTech Authorship	First
Abstract	本发明提供爪蛙嵌合体及其制备方法。该爪蛙嵌合体的制备方法包括以下步骤：将荧光蛋白编码序列导入热带爪蛙受精卵，构建荧光蛋白标记的转基因热带爪蛙；将转基因热带爪蛙传代，得到荧光蛋白标记的热带爪蛙胚胎；将热带爪蛙胚胎的细胞转入非洲爪蛙囊胚腔，形成嵌合胚胎。本发明实施例所提供的制备方法将转基因技术与异种移植技术相结合，将荧光蛋白的编码序列转入热带爪蛙受精卵，得到转基因热带爪蛙，传代后获得的荧光蛋白标记的转基因热带爪蛙胚胎的细胞移植进非洲爪蛙胚胎的囊胚腔中，可以借助荧光显微镜观察热带爪蛙细胞在非洲爪蛙胚胎里的发育情况。转基因方式得到的嵌合胚胎的单细胞内的荧光强度较为稳定，可以进行长期的追踪观察。
Other Abstract	The invention provides a clawed frog chimera and a preparation method thereof. The preparation method of the clawed frog chimera comprises the following steps of: introducing a fluorescent protein coding sequence into a fertilized egg of a xenopus tropicalis to construct a fluorescent protein labelled transgenic xenopus tropicalis; carrying out passage on the transgenic xenopus tropicalis to obtain a fluorescent protein labelled xenopus tropicalis embryo; and, transferring cells of the xenopus tropicalis embryo into the blastocyst cavity of a xenopus laevis to form a chimeric embryo. Accordingto the preparation method provided by the embodiment of the invention, a transgenic technology and a xenograft technology are combined; the fluorescent protein coding sequence is transferred into thefertilized egg of the xenopus tropicalis to obtain the transgenic xenopus tropicalis; the cells of the fluorescent protein labelled transgenic xenopus tropicalis embryo obtained after passage are transplanted into the blastocyst cavity of a xenopus laevis embryo; the development condition of the cells of the xenopus tropicalis in the xenopus laevis embryo can be observed by virtue of a fluorescence microscope; the fluorescence intensity in a single cell of the chimeric embryo obtained in a transgenic mode is relatively stable; and long-term tracking observation can be carried out.
CPC Classification Number	C12N15/8509 ; C12N15/65 ; C12N15/89 ; A01K67/0275 ; A01K67/0271 ; A01K2207/05 ; A01K2207/12 ; A01K2227/50 ; A01K2267/0393
IPC Classification Number	C12N15/85 ; C12N15/65 ; C12N15/89 ; A01K67/027
INPADOC Legal Status	（ENTRY INTO FORCE OF REQUEST FOR SUBSTANTIVE EXAMINATION）[2021-01-22][CN]
INPADOC Patent Family Count	1
Extended Patent Family Count	1
Priority date	2020-09-07
Patent Agent	刘燚
Agency	广州嘉权专利商标事务所有限公司
URL	[Source Record]
Data Source	PatSnap
Document Type	Patent
Identifier	http://kc.sustech.edu.cn/handle/2SGJ60CL/534387
Department	Department Of Chemical Biology And Quantitative Biology
Recommended Citation GB/T 7714	陈永龙,李兰心,石照应. 爪蛙嵌合体及其制备方法.

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